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The book reviews the latest experimental results of charm and bottom flavor physics at the Tevatron proton-antiproton collider. The measurements of lifetimes, branching ratios and mixing properties of heavy flavored hadrons provide important constraints on fundamental parameters of the standard model – the elements of the CKM matrix. Comparisons of experimental results with theoretical predictions allow to search for physics beyond the standard model or to set bounds on parameters of new physics models. The experimental techniques developed at the Tevatron are highly relevant for the next generation flavor physics experiments at the LHC.