Overview
- A comprehensive text that starts from the fundamental optical imaging concepts
- Guides the readers to the most advanced methods in fluorescence imaging and super-resolution techniques
- An essential text for courses in imaging optics, photophysics, fluorescence microscopy and imaging, methods in cell biology
- A thorough introduction to fluorescence microscopy and imaging for students in biophysics, molecular and cell biology, biomedical engineering, applied physics
- Contains three useful Appendices
- Lists of recommended books at the end of each chapter, to expand on various specialized topics
- Written by two acknowledged experts in this rapidly evolving, interdisciplinary field
- Includes supplementary material: sn.pub/extras
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Table of contents (11 chapters)
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Front Matter
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Basics and Fundamentals
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Front Matter
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Back Matter
Keywords
- Advanced fluorescence microscopy techniques
- Advances in fluorescence spectroscopy and microscopy
- Fluorophores and fluorescent probes
- Fourier, Fresnel, Fraunhofer geometrical optics
- Imaging optics
- Molecular physics and photophysics
- Quantitative fluorescence microscopy
- Ray optics and wave optics
- Super-resolution microscopy
- Textbook on fluorescence imaging
- Biological Microscopy
About this book
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Reviews
Authors and Affiliations
About the authors
Alberto Diaspro, born in Genoa, Italy, on April 7, 1959, received his Laurea in Electronic Engineering in 1983, Univ.of Genoa. Dir. Nanophys at Italian Institute of Technology (IIT), Deputy Director of IIT, Prof. Appl. Phys. at Univ.of Genoa. President of Optics Within Life Sciences, IEEE Senior member and Editor in chief of Microscopy Research and Technique (7/2013).
Alberto Diaspro is a member of the Bioengineering and Robotics PhD Advisory Committee (University of Genova) and affiliated with the Institute of Biophysics of the National Research Council (CNR). He founded LAMBS in 2003 (Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy, www.lambs.it). President of EBSA (European Biophysical Societies' Association, www.ebsa.org) (2009?2011), President of OWLS (Optics with Life Sciences) (2010?2014) and member of the International Relations Committee of the Biophysical Society. ISI Pubs > 250, H=30, cit > 4000 (Google Scholar). AD isinvolved in research development of instrumentation for applications in biophysics and biomedical engineering and in nanobiotechnological projects.
Partha Pratim Mondal is an assistant professor at the Indian Institute of Science in the department of Instrumentation and Applied Physics. He received his Ph.D. in Physics from the Indian Institute of Science, Bangalore, India in 2005. He has been a researcher at the MIT, Cambridge, MA, USA and at the universities of Genova, Italy and also was a ICTP Fellow, Trieste, Italy. He has more than 30 journal publications to his name and 18 contributions to conference proceedings. Currently, he is Associate Editor for Scientific Reports (Nature Publishing) , Academic Editor to the AIP Advances journal and an editorial board member to ‘Microscopy, Research and Technique (Wiley).
Bibliographic Information
Book Title: Fundamentals of Fluorescence Microscopy
Book Subtitle: Exploring Life with Light
Authors: Partha Pratim Mondal, Alberto Diaspro
DOI: https://doi.org/10.1007/978-94-007-7545-9
Publisher: Springer Dordrecht
eBook Packages: Physics and Astronomy, Physics and Astronomy (R0)
Copyright Information: Springer Science+Business Media Dordrecht 2014
Hardcover ISBN: 978-94-007-7544-2Published: 08 January 2014
Softcover ISBN: 978-94-017-7966-1Published: 23 August 2016
eBook ISBN: 978-94-007-7545-9Published: 12 December 2013
Edition Number: 1
Number of Pages: XV, 218
Number of Illustrations: 85 b/w illustrations, 55 illustrations in colour
Topics: Spectroscopy and Microscopy, Biological Microscopy, Biological and Medical Physics, Biophysics, Optics, Lasers, Photonics, Optical Devices, Spectroscopy/Spectrometry