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A comprehensive text that starts from the fundamental optical imaging concepts
Guides the readers to the most advanced methods in fluorescence imaging and super-resolution techniques
An essential text for courses in imaging optics, photophysics, fluorescence microscopy and imaging, methods in cell biology
A thorough introduction to fluorescence microscopy and imaging for students in biophysics, molecular and cell biology, biomedical engineering, applied physics
Contains three useful Appendices
Lists of recommended books at the end of each chapter, to expand on various specialized topics
Written by two acknowledged experts in this rapidly evolving, interdisciplinary field
This book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging.
Content Level »Graduate
Keywords »Advanced fluorescence microscopy techniques - Advances in fluorescence spectroscopy and microscopy - Fluorophores and fluorescent probes - Fourier, Fresnel, Fraunhofer geometrical optics - Imaging optics - Molecular physics and photophysics - Quantitative fluorescence microscopy - Ray optics and wave optics - Super-resolution microscopy - Textbook on fluorescence imaging