Logo - springer
Slogan - springer

Physics - Optics & Lasers | Fundamentals of Fluorescence Microscopy

Fundamentals of Fluorescence Microscopy

Exploring Life with Light

Mondal, Partha Pratim, Diaspro, Alberto

2014, XV, 218 p. 140 illus., 55 illus. in color.

Available Formats:

Springer eBooks may be purchased by end-customers only and are sold without copy protection (DRM free). Instead, all eBooks include personalized watermarks. This means you can read the Springer eBooks across numerous devices such as Laptops, eReaders, and tablets.

You can pay for Springer eBooks with Visa, Mastercard, American Express or Paypal.

After the purchase you can directly download the eBook file or read it online in our Springer eBook Reader. Furthermore your eBook will be stored in your MySpringer account. So you can always re-download your eBooks.


(net) price for USA

ISBN 978-94-007-7545-9

digitally watermarked, no DRM

Included Format: PDF and EPUB

download immediately after purchase

learn more about Springer eBooks

add to marked items


Hardcover version

You can pay for Springer Books with Visa, Mastercard, American Express or Paypal.

Standard shipping is free of charge for individual customers.


(net) price for USA

ISBN 978-94-007-7544-2

free shipping for individuals worldwide

usually dispatched within 3 to 5 business days

add to marked items

  • A comprehensive text that starts from the fundamental optical imaging concepts
  • Guides the readers to the most advanced methods in fluorescence imaging and super-resolution techniques
  • An essential text for courses in imaging optics, photophysics, fluorescence microscopy and imaging, methods in cell biology
  • A thorough introduction to fluorescence microscopy and imaging for students in biophysics, molecular and cell biology, biomedical engineering, applied physics
  • Contains three useful Appendices
  • Lists of recommended books at the end of each chapter, to expand on various specialized topics
  • Written by two acknowledged experts in this rapidly evolving, interdisciplinary field

This book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors.

The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence  factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices.

The book is organized into two parts. The first part deals with basics of imaging optics and its applications.  The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging.

Content Level » Graduate

Keywords » Advanced fluorescence microscopy techniques - Advances in fluorescence spectroscopy and microscopy - Fluorophores and fluorescent probes - Fourier, Fresnel, Fraunhofer geometrical optics - Imaging optics - Molecular physics and photophysics - Quantitative fluorescence microscopy - Ray optics and wave optics - Super-resolution microscopy - Textbook on fluorescence imaging

Related subjects » Analytical Chemistry - Biophysics & Biological Physics - Life Sciences - Optics & Lasers

Table of contents / Preface / Sample pages 

Distribution rights 

Distribution rights for India: Delhi Book Store, New Delhi, India

Popular Content within this publication 



Read this Book on Springerlink

Services for this book

New Book Alert

Get alerted on new Springer publications in the subject area of Spectroscopy and Microscopy.