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Summarizes the current top state-of-the-art in the theory and experimental realisation
Foreword by a Nobel Laureate
Contributing authors are the top scientists in this field
Many examples and practical application results
Useful reference to researchers and graduate students alike
Written by the leading experts in the field, this book describes the development and current state-of-the-art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.
Content Level »Research
Keywords »Dynamics of single molecules - Fluorescence correlation spectroscopy - Quantum dots - Single molecule spectroscopy - Spectroscopy in nanoscience - diffraction - imaging - microscopy - molecule - nanostructure - quantum dot - spectroscopy
Introductory Lecture: Molecular Dynamics of Single Molecules.- How Biomolecular Motors Work: Synergy Between Single Molecule Experiments and Single Molecule Simulations.- Detection of Single Molecules and Single Molecule Processes.- Single-Molecule Optical Spectroscopy and Imaging: From Early Steps to Recent Advances.- Single Molecules as Optical Probes for Structure and Dynamics.- FCS and Single Molecule Spectroscopy.- Fluorescence Correlation Spectroscopy.- Single Molecule Spectroscopy Illuminating the Molecular Dynamics of Life.- Chemical Fluxes in Cellular Steady States Measured by Fluorescence Correlation Spectroscopy.- In Vivo Fluorescence Correlation and Cross-Correlation Spectroscopy.- Fluorescence Flicker as a Read-out in FCS: Principles, Applications and Further Developments.- Quantum Dots and Single Molecule Behaviour.- Development of Nanocrystal Molecules for Plasmon Rulers and Single Molecule Biological Imaging.- Size-Minimized Quantum Dots for Molecular and Cellular Imaging.- Mapping Transcription Factors on Extended DNA: A Single Molecule Approach.- Molecular Motion of Contractile Elements and Polymer Formation.- Single Molecule Measurement, a Tool for Exploring the Dynamic Mechanism of Biomolecules.- Viral DNA Packaging: One Step at a Time.- Chemo-Mechanical Coupling in the Rotary Molecular Motor F-ATPase.- Force and Multiparameter Spectroscopy on Functional Active Proteins.- Mechanoenzymatics and Nanoassembly of Single Molecules.- Single Cell Physiology.- Force-Clamp Spectroscopy of Single Proteins.- Unraveling the Secrets of Bacterial Adhesion Organelles Using Single-Molecule Force Spectroscopy.- Nanoscale Microscopy and High Resolution Imaging.- Far-Field Optical Nanoscopy.- Sub-Diffraction-Limit Imaging with Stochastic Optical Reconstruction Microscopy.- Assessing Biological Samples with Scanning Probes.- Single Molecule Microscopy in Individual Cells.- Enzymology and Life at the Single Molecule Level.- Controlling Chemistry in Dynamic Nanoscale Systems.- Catalysis of Single Enzyme Molecules.- Single-Molecule Protein Conformational Dynamics in Enzymatic Reactions.- Watching Individual Enzymes at Work.- The Influence of Symmetry on the Electronic Structure of the Photosynthetic Pigment-Protein Complexes from Purple Bacteria.- New Fields and Outlook.- Exploring Nanostructured Systems with Single-Molecule Probes: From Nanoporous Materials to Living Cells.- Gene Regulation: Single-Molecule Chemical Physics in a Natural Context.