Detection of Non-Amplified Genomic DNA

1. Front Matter

   Pages i-xiv

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2. DNA Analysis: Current Issues

   1. Front Matter

      Pages 1-1

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3. DNA analysis: current issues

   1. Genetic Analyses in Health Laboratories: Current Status and Expectations

      • Alessia Finotti, Giulia Breveglieri, Monica Borgatti, Roberto Gambari

      Pages 3-24

   2. DNA Analyses in Food Safety and Quality: Current Status and Expectations

      • Rosangela Marchelli, Tullia Tedeschi, Alessandro Tonelli

      Pages 25-63

4. Advanced Materials for Ultrasensitive Analysis

   1. Front Matter

      Pages 65-65

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5. Advanced materials for ultrasensitive analysis

   1. Engineered Nanostructures for the Ultrasensitive DNA Detection

      • Dong-Kwon Lim, Amit Kumar, Jwa-Min Nam

      Pages 67-87

   2. Advanced Molecular Probes for Sequence-Specific DNA Recognition

      • Alessandro Bertucci, Alex Manicardi, Roberto Corradini

      Pages 89-124

   3. Charged Conjugated Polymers

      • Fengting Lv, Shu Wang

      Pages 125-150

6. Detection Strategies – Sensors

   1. Front Matter

      Pages 151-151

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7. Detection strategies - Sensors

   1. Optical Detection of Non-amplified Genomic DNA

      • Di Li, Chunhai Fan

      Pages 153-183

   2. Electrochemical Detection of DNA Using Nanomaterials Based Sensors

      • Sergio Marín, Arben Merkoçi

      Pages 185-201

   3. Piezoelectric Sensing for Sensitive Detection of DNA

      • Maria Minunni

      Pages 203-233

   4. Surface Plasmon Resonance-Based Methods

      • Roberta D’Agata, Giuseppe Spoto

      Pages 235-261

   5. Parallel Optical and Electrochemical DNA Detection

      • Wolfgang Knoll, Jianyun Liu, Lifang Niu, Peter Eigil Nielsen, Louis Tiefenauer

      Pages 263-278

8. Detection Strategies – Sequencing

   1. Front Matter

      Pages 279-279

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9. Detection strategies - Sequencing

   1. Recent Advances in Sequencing Technology

      • John F. Thompson, Fatih Ozsolak, Patrice M. Milos

      Pages 281-308

10. Back Matter

    Pages 309-315

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This book offers an overview of state-of-the-art in non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in this field.

• Enhanced sensitivity in DNA detection
• How to perform gene sequencing
• PCR-free DNA analysis

• , Department of Chemical Science, University of Catania, Catania, Italy

  Giuseppe Spoto

• , Faculty of Science, University of Parma, Parma, Italy

  Roberto Corradini

Prof. Giuseppe Spoto, University of Catania, Catania, Italy

Prof. Roberto Corradini, University of Parma, Parma, Italy

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