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Physics - Biophysics & Biological Physics | Handbook of Single-Molecule Biophysics

Handbook of Single-Molecule Biophysics

Hinterdorfer, Peter, van Oijen, Antoine (Eds.)

2009, XXI, 626 p.

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  • Describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping
  • Addresses the use of single-molecule techniques in super-resolution and functional imaging
  • Includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells
  • Integrates single-molecule biophysics and nanoscience

The last decade has seen the development of a number of novel biophysical methods that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at this fundamental level of sensitivity has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them. Coverage includes:

  • Experimental techniques to monitor and manipulate individual biomolecules
  • The use of single-molecule techniques in super-resolution and functional imaging
  • Single-molecule studies of physical properties of biomolecules
  • Single-molecule enzymology and biochemistry
  • Single molecules in the membrane
  • Single-molecule techniques in living cells
  • Integration of single-molecule biophysics and nanoscience

Content Level » Research

Keywords » Biomembran - Biophysics - FRET - cells - fluorescence - imaging - microscopy - molecule - proteins

Related subjects » Biomedical Engineering - Biophysics & Biological Physics - Life Sciences - Molecular Medicine - Nanotechnology - Optics & Lasers

Table of contents 

Chapter 1 - Single-molecule fluorescence tracking Ahmet Yildiz (UCSF) Chapter 2 - Single molecule studies in the membrane Gerhard Schuetz (Linz) Thomas Schmidt (Leiden) Chapter 3 - Single-molecule imaging in live cells Jie Xiao (Johns Hopkins) Chapter 4 - Superresolution imaging Xiaowei Zhuang (Harvard) Chapter 5 - Fluorescence Resonance Energy Transfer Achillefs Kapanidis (Oxford) Chapter 6 - Single-molecule enzymology Antoine van Oijen (Harvard) Chapter 7 - Rotary proteins Richard Berry (Oxford) Chapter 8 - Fluorescence Correlation Spectroscopy Petra Schwille (Dresden) Chapter 9 - Advanced Concepts of Fluorescence Fluctuation Spectroscopy Jörg Enderlein (Tübingen) Chapter 10 - Nucleic-acid analysis at the single-molecule level Amit Meller (Boston University) Chapter 11 - Nanopores: Geneneration and Single-Molecule Applications Stefan Howorka (UCL) Zuzanna Siwy (UC-Irvine) Chapter 12 - Optical trapping Michael Woodside (Alberta) Megan Valentine (UCSB) Chapter 13 - Magnetic tweezing Nynke Dekker (Delft) Chapter 14 - Protein/DNA unfolding Matthias Rief (Munich) Chapter 15 - Single molecule recognition Ziv Reich (Weizman) Peter Hinterdorfer (Linz) Chapter 16 - Nano-scale AFM imaging Simon Scheuring (Institut Curie) Daniel J. Müller (Dresden) Chapter 17 - High Speed AFM T. Ando (Kanazawa) Chapter 18 - Recognition imaging Yves Dufrene (Leuven) Peter Hinterdorfer (Linz) Chapter 19 - Atomic force microscopy of protein-protein interactions Vincent Moy (Miami) Chapter 20 - A New Approach to Analysis of Single MoleculeForce Measurements Evan Evans (Vancouver) Chapter 21 - Single Molecule Recognition: Extracting Information from Individual Binding Events and Their Correlation Cheng Zhu (Georgia Tech)

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