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Handbook of Single-Molecule Biophysics

  • Book
  • © 2009

Overview

Editors:
  1. Peter Hinterdorfer

    1. Institut für Biophysik, Universität Linz, Linz, Austria

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  2. Antoine Oijen

    1. Harvard Medical School, Harvard University, Boston, U.S.A.

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    You can also search for this editor in PubMed   Google Scholar

  • Describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping

  • Addresses the use of single-molecule techniques in super-resolution and functional imaging

  • Includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells

  • Integrates single-molecule biophysics and nanoscience

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Table of contents (21 chapters)

  1. Front Matter

    Pages i-xxi
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  2. Single-Molecule Fluorescent Particle Tracking

    • Ahmet Yildiz
    Pages 1-18

  3. Single-Molecule Analysis of Biomembranes

    • Thomas Schmidt, Gerhard J. Schütz
    Pages 19-42

  4. Single-Molecule Imaging in Live Cells

    • Jie Xiao
    Pages 43-93

  5. Fluorescence Imaging at Sub-Diffraction-Limit Resolution with Stochastic Optical Reconstruction Microscopy

    • Graham T. Dempsey, Wenqin Wang, Xiaowei Zhuang
    Pages 95-127

  6. Single-Molecule FRET: Methods and Biological Applications

    • Ling Chin Hwang, Johannes Hohlbein, Seamus J. Holden, Achillefs N. Kapanidis
    Pages 129-163

  7. Single-Molecule Enzymology

    • Joseph J. Loparo, Antoine van Oijen
    Pages 165-182

  8. Single-Molecule Studies of Rotary Molecular Motors

    • Teuta Pilizota, Yoshiyuki Sowa, Richard M. Berry
    Pages 183-216

  9. Fluorescence Correlation Spectroscopy in Living Cells

    • Thomas Weidemann, Petra Schwille
    Pages 217-241

  10. Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy

    • Jörg Enderlein
    Pages 243-263

  11. Single-Molecule Studies of Nucleic Acid Interactions Using Nanopores

    • Meni Wanunu, Gautam V. Soni, Amit Meller
    Pages 265-291

  12. Nanopores: Generation, Engineering, and Single-Molecule Applications

    • Stefan Howorka, Zuzanna Siwy
    Pages 293-339

  13. Single-Molecule Manipulation Using Optical Traps

    • Michael T. Woodside, Megan T. Valentine
    Pages 341-370

  14. Magnetic Tweezers for Single-Molecule Experiments

    • I. D. Vilfan, J. Lipfert, D. A. Koster, S. G. Lemay, N. H. Dekker
    Pages 371-395

  15. Folding of Proteins under Mechanical Force

    • Michael Schlierf, Matthias Rief
    Pages 397-406

  16. Probing the Energy Landscape of Protein-Binding Reactions by Dynamic Force Spectroscopy

    • Andreas Ebner, Reinat Nevo, Christian Ranki, Johannes Preiner, Hermann Gruber, Ruti Kapon et al.
    Pages 407-447

  17. Probing Single Membrane Proteins by Atomic Force Microscopy

    • S. Scheuring, K. Tanuj Sapra, Daniel J. Müller
    Pages 449-485

  18. High-Speed Atomic Force Microscopy

    • Toshio Ando, Takayuki Uchihashi
    Pages 487-523

  19. Recognition Imaging Using Atomic Force Microscopy

    • Andreas Ebner, Lilia Chtcheglova, Jilin Tang, David Alsteens, Vincent Dupres, Yves F. Dufrêne et al.
    Pages 525-554

  20. Atomic Force Microscopy of Protein–Protein Interactions

    • Xiaohui Zhang, Felix Rico, Amy J. Xu, Vincent T. Moy
    Pages 555-570
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Keywords

About this book

During the last decade, a number of novel biophysical methods have been developed that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at the fundamental level of sensitivity, that of a single molecule, has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them.

Editorial Advisory Board: Daniel Müller, Cheng Zhu, Claus Seidel, Xiaowei Zhuang, Thomas Schmidt, Nynke Dekker.

Reviews

From the reviews: “The Handbook of Single-Molecule Biophysics, edited by Hinterdorfer (Johannes Kepler Univ. of Linz, Austria) and van Oijen (Harvard Medical School), contains 21 chapters that address various aspects of techniques used to investigate single-molecule biophysics. The chapters, many authored by leading experts in their respective fields, cover specialized biophysical techniques, their applications, background into the technical design, and practical aspects of implementing these approaches. … Summing Up: Recommended. Graduate students, researchers, faculty, and professionals.” (C. A. Reinhart-King, Choice, Vol. 47 (10), June, 2010)

Editors and Affiliations

  • Institut für Biophysik, Universität Linz, Linz, Austria

    Peter Hinterdorfer

  • Harvard Medical School, Harvard University, Boston, U.S.A.

    Antoine Oijen

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