Editors:
Describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping
Addresses the use of single-molecule techniques in super-resolution and functional imaging
Includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells
Integrates single-molecule biophysics and nanoscience
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Table of contents (21 chapters)
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Front Matter
About this book
During the last decade, a number of novel biophysical methods have been developed that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at the fundamental level of sensitivity, that of a single molecule, has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them.
Editorial Advisory Board: Daniel Müller, Cheng Zhu, Claus Seidel, Xiaowei Zhuang, Thomas Schmidt, Nynke Dekker.
Reviews
Editors and Affiliations
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Institut für Biophysik, Universität Linz, Linz, Austria
Peter Hinterdorfer
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Harvard Medical School, Harvard University, Boston, U.S.A.
Antoine Oijen
Bibliographic Information
Book Title: Handbook of Single-Molecule Biophysics
Editors: Peter Hinterdorfer, Antoine Oijen
DOI: https://doi.org/10.1007/978-0-387-76497-9
Publisher: Springer New York, NY
eBook Packages: Physics and Astronomy, Physics and Astronomy (R0)
Copyright Information: Springer-Verlag New York 2009
Hardcover ISBN: 978-0-387-76496-2Published: 24 July 2009
Softcover ISBN: 978-1-4939-3919-0Published: 23 August 2016
eBook ISBN: 978-0-387-76497-9Published: 24 December 2009
Edition Number: 1
Number of Pages: XXI, 626
Topics: Biological and Medical Physics, Biophysics, Life Sciences, general, Molecular Medicine, Optics, Lasers, Photonics, Optical Devices, Biomedical Engineering and Bioengineering, Nanotechnology