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New & Forthcoming Titles | Multiheme Cytochromes

Multiheme Cytochromes

Salgueiro, Carlos A.

2015, 50 p. 20 illus.

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ISBN 978-3-642-44961-1

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  • About this book

  • Provides a snapshot of the key concepts, methodology and applications of multiheme cyctochromes
  • Outlines the great potential for multiheme cytochromes in bioremediation and bioenergy applications
  • Provides readers with an overview of the latest research in multiheme cytochromes in the last few years
  •  
Multiheme Cytochromes introduces the structural and functional characterization of this important group of proteins. The content of each chapter is aimed at the non-specialist so that key concepts, methodologies and applications can be presented in a "snapshot" style volume. Multiheme cytochromes are ever more important now that it is possible to obtain genome sequences of microorganisms which have major biotechnological and environmental implications. There is a tremendous profusion of multiheme cytochromes which have great potential as targets for bioremediation and bioenergy applications.

Content Level » Research

Keywords » Bioremediation and Microbial Electricity Production - Electron Transfer - Energy Transduction - Multiheme Cytochromes - NMR of Proteins - Protein Structure - Redox Properties - Redox-Bohr Effect - Structural-Functional Relationship - Thermodynamic Characterization

Related subjects » Organic Chemistry

Table of contents 

Multiheme cytochromes.- Introduction: brief description, spectroscopic properties, function and biological context.- Protein sources: microorganisms as sources of multiheme cytochromes, heterologous versus non-heterologous production.- Protein purification: initial recovery of multiheme cytochromes, removal of whole cells and debris, concentration and primary purification, column chromatography.- Structural characterization of multiheme cytochromes.- Introduction: structural, binding and dynamics studies, NMR-based methodologies.- Sample preparation: non-isotopic labeled versus isotopic labeled proteins.- Overview of protein structure: solution and X-ray crystal structures.- Interaction and dynamic studies: docking sites mapping, identification of redox partners.- Recent advances in structural characterization.- Functional characterization of multiheme cytochromes.- Introduction: redox equilibria, macroscopic versus microscopic redox properties.- Thermodynamic parameters: redox and redox-Bohr centres, redox potentials, redox-interactions and redox-Bohr interactions.- Functional and physiological outcome: preferential electron transfer pathways, energy transduction mechanisms.- Recent advances in functional characterization.- Multiheme cytochromes as targets for practical applications.- Introduction: microbial respiratory diversity.- Multiheme cytochromes and extracellular electron transfer.- Bioremediation and electricity harvesting applications: removal of toxic and heavy metals from environment, microbial fuel cells.

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