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New & Forthcoming Titles | Membrane Proteins - Folding, Association, and Design

Membrane Proteins

Folding, Association, and Design

Series: Methods in Molecular Biology, Vol. 1063

Ghirlanda, Giovanna, Senes, Alessandro (Eds.)

2013, X, 246 p. 61 illus., 37 illus. in color.

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  • Focuses on model systems for the study of structure, folding, and association of proteins in the membrane
  • Presents laboratory-ready protocols provided by experts in the field
  • Features key tips and implementation advice to ensure successful results

Focusing on model systems for the study of structure, folding, and association in the membrane, Membrane Proteins: Folding, Association, and Design presents an overview of methods that can be applied to these intricate systems. The volume is divided into four detailed sections, covering association of transmembrane helices, interactions with the lipid bilayer, NMR methods, as well as a variety of engineering approaches.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Membrane Proteins: Folding, Association, and Design serves as an ideal guide for researchers reaching for the tantalizing possibility of designing novel membrane proteins with tailored functionality.

Content Level » Professional/practitioner

Keywords » Functional artificial proteins - Lipid bilayer - NMR methods - Protein engineering - Protein folding - Protein-protein interactions - Transmembrane peptides

Related subjects » Cell Biology

Table of contents 

Part I: Association of Transmembrane Helices


1. Use of Thiol-Disulfide Exchange Method to Study Transmembrane Peptide Association in Membrane Environments

            Lidia Cristian and Yao Zhang


2. Measurement of Transmembrane Peptide Interactions in Liposomes Using Förster Resonance Energy Transfer (FRET)

            Ambalika Khadria and Alessandro Senes


3. Measuring Transmembrane Helix Interaction Strengths in Lipid Bilayers Using Steric Trapping

            Heedeok Hong, Yu-Chu Chang, and James U. Bowie


4. Genetics Systems for Monitoring Interactions of Transmembrane Domains in Bacterial Membranes

            Lydia Tome, Dominik Steindorf, and Dirk Schneider


Part II: Interactions with the Lipid Bilayer


5. Analyzing the Effects of Hydrophobic Mismatch on Transmembrane α-Helices Using Tryptophan Fluorescence Spectroscopy

            Gregory A. Caputo


6. Folding Alpha Helical Membrane Proteins into Liposomes In Vitro and Determination of Secondary Structure

            Heather E. Findlay and Paula J. Booth


7. Solvation Models and Computational Prediction of Orientations of Peptides and Proteins in Membranes

            Andrei L. Lomize and Irina D. Pogozheva


Part III: NMR Methods


8. Membrane Protein Structure Determination: Back to the Membrane

            Yong Yao, Yi Ding, Ye Tian, Stanley J. Opella, and Francesca M. Marassi


9. On the Role of NMR Spectroscopy for Characterization of Antimicrobial Peptides

            Fernando Porcelli, Ayyalusamy Ramamoorthy, George Barany, and Gianluigi Veglia


Part IV: Engineering Approaches


10. Prediction and Design of Outer Membrane Protein-Protein Interactions

            Vikas Nanda, Daniel Hsieh, and Alexander Davis


11. Design of Transmembrane Peptides: Coping with Sticky Situations

            Arianna Rath and Charles M. Deber


12. Engineering and Utilization of Reporter Cell Lines for Cell-Based Assays of Transmembrane Receptors

            Matthew W. Lluis and Hang Yin


13. Fluorination in the Design of Membrane Protein Assemblies

            Vijay M. Krishnamurthy and Krishna Kumar

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