2013, XIII, 586 p. 151 illus., 63 illus. in color.
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Covers the entire range of techniques used in electron crystallography, from protein sample preparation to modeling of conformational changes
Features detailed protocols suitable for labs with minimal equipment and subject familiarity and other techniques for the most state-of-the-art facilities
Includes key tips and expert implementation advice to ensure successful results
The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well developed and has produced a number of atomic models of membrane proteins in a lipid environment. Focused on comprehensive experimental protocols, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols covers the entire range of techniques used in electron crystallography, including protein sample preparation, 2D crystallization, and screening in negative stain over electron cryo-microscopy (cryo-EM) and data processing, as well as modeling of conformational changes. Additional chapters provide perspective on past, present, and future challenges as well as complementary methods. Written for the popular Methods in Molecular Biology™ series, the work contains the kind of detailed descriptions and implementation advice necessary to ensure successful results.
Comprehensive and cutting-edge, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols serves laboratories new to the methods as well as state-of-the-art facilities pursuing this exciting area of protein science.
Content Level »Professional/practitioner
Keywords »3D density map - Electron cryo-cryomicroscopy - Electron crystallography - Negative stain - Protein sample preparation - Two-dimensional (2D) crystals - X-ray crystallography