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Provides a snapshot of the key concepts, methodology and applications of multiheme cyctochromes
Outlines the great potential for multiheme cytochromes in bioremediation and bioenergy applications
Provides readers with an overview of the latest research in multiheme cytochromes in the last few years
Multiheme Cytochromes introduces the structural and functional characterization of this important group of proteins. The content of each chapter is aimed at the non-specialist so that key concepts, methodologies and applications can be presented in a "snapshot" style volume. Multiheme cytochromes are ever more important now that it is possible to obtain genome sequences of microorganisms which have major biotechnological and environmental implications. There is a tremendous profusion of multiheme cytochromes which have great potential as targets for bioremediation and bioenergy applications.
Content Level »Research
Keywords »Bioremediation and Microbial Electricity Production - Electron Transfer - Energy Transduction - Multiheme Cytochromes - NMR of Proteins - Protein Structure - Redox Properties - Redox-Bohr Effect - Structural-Functional Relationship - Thermodynamic Characterization
Multiheme cytochromes.- Introduction: brief description, spectroscopic properties, function and biological context.- Protein sources: microorganisms as sources of multiheme cytochromes, heterologous versus non-heterologous production.- Protein purification: initial recovery of multiheme cytochromes, removal of whole cells and debris, concentration and primary purification, column chromatography.- Structural characterization of multiheme cytochromes.- Introduction: structural, binding and dynamics studies, NMR-based methodologies.- Sample preparation: non-isotopic labeled versus isotopic labeled proteins.- Overview of protein structure: solution and X-ray crystal structures.- Interaction and dynamic studies: docking sites mapping, identification of redox partners.- Recent advances in structural characterization.- Functional characterization of multiheme cytochromes.- Introduction: redox equilibria, macroscopic versus microscopic redox properties.- Thermodynamic parameters: redox and redox-Bohr centres, redox potentials, redox-interactions and redox-Bohr interactions.- Functional and physiological outcome: preferential electron transfer pathways, energy transduction mechanisms.- Recent advances in functional characterization.- Multiheme cytochromes as targets for practical applications.- Introduction: microbial respiratory diversity.- Multiheme cytochromes and extracellular electron transfer.- Bioremediation and electricity harvesting applications: removal of toxic and heavy metals from environment, microbial fuel cells.