Overview
- Editors:
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Joel P. Mackay
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School of Molecular &, Microbial Biosciences, University of Sydney, Sydney, Australia
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David J. Segal
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Dept. Pharmacology & Genome Center, University of California, Davis, Davis, USA
- Chapters from nearly all of the top research groups in the field
- Sections on zinc finger nucleases, one of the most exciting tools for gene therapy and genome engineering in the past decade
- Includes supplementary material: sn.pub/extras
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Table of contents (29 protocols)
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Engineering Zinc Finger Proteins
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- Mital S. Bhakta, David J. Segal
Pages 3-30
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- Stacey Thibodeau-Beganny, Morgan L. Maeder, J. Keith Joung
Pages 31-50
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- Andreu Alibés, Luis Serrano, Alejandro D. Nadra
Pages 77-88
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Artificial Transcription Factors
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- Kimberley A. Hoeksema, D. Lorne J. Tyrrell
Pages 97-116
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- Sabine Stolzenburg, Alan Bilsland, W. Nicol Keith, Marianne G. Rots
Pages 117-132
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- Seokjoong Kim, Eun Ji Kim, Jin-Soo Kim
Pages 133-147
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- Renata Z. Jurkowska, Albert Jeltsch
Pages 149-161
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- Adriana S. Beltran, Pilar Blancafort
Pages 163-182
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- Claudio Passananti, Nicoletta Corbi, Annalisa Onori, Maria Grazia Di Certo, Elisabetta Mattei
Pages 183-206
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Zinc Finger Nucleases
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Front Matter
Pages 207-207
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- Vardit Zeevi, Andriy Tovkach, Tzvi Tzfira
Pages 209-225
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- Toni Cathomen, Cem Şöllü
Pages 227-235
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- Tatjana I. Cornu, Toni Cathomen
Pages 237-245
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- Dmitry Y. Guschin, Adam J. Waite, George E. Katibah, Jeffrey C. Miller, Michael C. Holmes, Edward J. Rebar
Pages 247-256
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About this book
Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications.
Editors and Affiliations
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School of Molecular &, Microbial Biosciences, University of Sydney, Sydney, Australia
Joel P. Mackay
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Dept. Pharmacology & Genome Center, University of California, Davis, Davis, USA
David J. Segal