Logo - springer
Slogan - springer

New & Forthcoming Titles | Structural Proteomics - High-Throughput Methods

Structural Proteomics

High-Throughput Methods

Series: Methods in Molecular Biology, Vol. 426

Kobe, Bostjan, Guss, Mitchell, Huber, Thomas (Eds.)

2008

A product of Humana Press
Available Formats:
eBook
Information

Springer eBooks may be purchased by end-customers only and are sold without copy protection (DRM free). Instead, all eBooks include personalized watermarks. This means you can read the Springer eBooks across numerous devices such as Laptops, eReaders, and tablets.

You can pay for Springer eBooks with Visa, Mastercard, American Express or Paypal.

After the purchase you can directly download the eBook file or read it online in our Springer eBook Reader. Furthermore your eBook will be stored in your MySpringer account. So you can always re-download your eBooks.

 
$119.00

(net) price for USA

ISBN 978-1-60327-058-8

digitally watermarked, no DRM

Included Format: PDF and EPUB

download immediately after purchase


learn more about Springer eBooks

add to marked items

Hardcover
Information

Hardcover version

You can pay for Springer Books with Visa, Mastercard, American Express or Paypal.

Standard shipping is free of charge for individual customers.

 
$159.00

(net) price for USA

ISBN 978-1-58829-809-6

free shipping for individuals worldwide

usually dispatched within 3 to 5 business days


add to marked items

Softcover
Information

Softcover (also known as softback) version.

You can pay for Springer Books with Visa, Mastercard, American Express or Paypal.

Standard shipping is free of charge for individual customers.

 
$159.00

(net) price for USA

ISBN 978-1-61737-789-1

free shipping for individuals worldwide

usually dispatched within 3 to 5 business days


add to marked items

  • First book of protocols to cover techniques in a new and emerging field
  • Offers step-by-step methods and protocols for reproducible lab experiments
  • Each protocol has a section where the major researchers in the field offer tricks and hints of the trade

Structural genomics is a newly emerging field that has arisen following the successful footsteps of the major sequencing efforts generally bundled under the heading "genomics". In Structural Proteomics: High Throughput Methods, readers are provided with a current view of all aspects of the 'pipeline' that takes protein targets to structures and how these have been optimized. This volume includes chapters describing in-depth the individual steps in the Structural Genomics pipeline, as well as overviews of individual Structural Genomics initiatives. Topics include Target Selection for structural genomics, Protein structure modeling, High-throughput cloning, Mass Spectrometry modeling. Structural Proteomics: High Throughput Methods, encourages its readers to access further details on the methodologies in on-line resources as well as in cited literature making it an invaluable resource. Most methods in this important book are as amenable to small laboratories as to large consortia, and do not require major investments in facilities. This essential volume provides insight into the diversity of approaches adopted by different laboratories and proves that 'high throughput' is really the defining characteristic of structural genomics.

Content Level » Research

Keywords » Annotation - Escherichia coli - High-throughput cloning - Protein structure modeling - Proteomics - Structural genomics - Termination - biology - enzymes - genes - genome - membrane proteins - protein complexes - protein structure - proteins

Related subjects » Biochemistry & Biophysics - Cell Biology - Genetics & Genomics - Systems Biology and Bioinformatics

Table of contents 

Section 1: Protein target selection, bioinformatic approaches and data management Target selection for structural genomics: An Overview. Russell L. Marsden and Christine A. Orengo A General target selection method for crystallographic proteomics. Gautier Robin, Nathan P. Cowieson, Gregor Guncar, Jade Forwood, Pawel Listwan, David A. Hume, Bostjan Kobe, Jennifer L. Martin, Thomas Huber Target Selection: Triage in the Structural Genomics Battlefield. James Raftery. Data Management in Structural Genomics: An Overview. S. Haquin, E. Oeuillet, A. Pajon, M. Harris, A.T. Jones, H. van Tilbeurgh, J.L. Markley, Z. Zolnai, and A. Poupon Data deposition and annotation at the Worldwide Protein Data Bank. Shuchismita Dutta, Kyle Burkhardt, Ganesh J. Swaminathan, Takashi Kosada, Kim Henrick, Haruki Nakamura, Helen M. Berman Prediction of protein disorder. Zsuzsanna Dosztányi and Peter Tompa Protein domain prediction. Helgi Ingolfsson and Golan Yona Protein Structure Modeling With MODELLER. Narayanan Eswar, David Eramian, Ben Webb, Min-Yi Shen and Andrej Sali Section 2: Protein production High throughput cloning with restriction enzymes. Volker Sievert, Asgar Ergin and Konrad Büssow Automated recombinant protein expression screening in Escherichia coli. Didier Busso, Matthieu Stierlé, Jean-Claude Thierry and Dino Moras From no expression to high-level soluble expression in Escherichia coli by screening a library of the target protein with randomized N-terminus. Kyoung Hoon Kim, Jin Kuk Yang, Geoffrey S. Waldo, Thomas C. Terwilliger, and Se Won Suh Application of High-Throughput Methodologies to the Expression of Recombinant Proteins in E. coli. YoavPeleg and Tamar Unger A high-throughput platform for eukaryotic genes. Yunjia Chen, Shihong Qiu, Chi-Hao Luan and Ming Luo High-Throughput production of recombinant human proteins for crystallography. Opher Gileadi, Nicola A. Burgess-Brown, Steve M. Colebrook, Georgina Berridge, Pavel Savitsky, Carol E.A. Smee, Peter Loppnau, Catrine Johansson, Eidarus Salah, Nadia H. Pantic Assembly of protein complexes by co-expression in prokaryotic and eukaryotic hosts. Anastassis Perrakis and Christophe Romier Cell-free protein synthesis for analysis by NMR spectroscopy. Margit A. Apponyi, Kiyoshi Ozawa, Nicholas E. Dixon and Gottfried Otting A medium or high-throughput protein refolding assay. Nathan P. Cowieson, Beth Wensley, Gautier Robin, Gregor Guncar, Jade Forwood, David A. Hume, Bostjan Kobe and Jennifer L. Martin Structural Proteomics of Membrane Proteins: A Survey of Published Techniques and Design of a Rational High-Throughput Strategy. Melissa Swope Willis and Christopher M. Koth Section 3: Biophysical and functional chatacterization of proteins Methods for protein characterization by mass spectrometry, thermal shift (ThermoFluor) assay, and multi-angle or static light scattering. Joanne E. Nettleship, James Brown, Matthew R. Groves and Arie Geerlof High-Throughput Methods for Analyzing Transition Metals in Proteins on a Microgram Scale. Anelia Atanassova, Martin Högbom and Deborah B. Zamble High Throughput Screening of Purified Proteins for Enzymatic Activity. Michael Proudfoot, Ekaterina Kuznetsova, Stephen A. Sanders, Claudio F. Gonzalez, Greg Brown, Aled M. Edwards, Cheryl H. Arrowsmith, Alexander F. Yakunin Section 4: Structural characterization of

Distribution rights 

Distribution rights for India: CBS Publishers, New Delhi, India

Popular Content within this publication 

 

Articles

Read this Book on Springerlink

Services for this book

New Book Alert

Get alerted on new Springer publications in the subject area of Protein Science.