Authors:
- Focuses on challenges in next generation sequencing technologies
- Challenges include sequencing errors, expensive processing and memory usage and sequencer specific errors
- This book provides overview on NGS methods, platforms and challenges and NGS sequence Assembly methods and challenges
- Includes supplementary material: sn.pub/extras
Part of the book series: SpringerBriefs in Systems Biology (BRIEFSBIOSYS, volume 7)
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Table of contents (11 chapters)
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Front Matter
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Introduction to Molecular Biology and Bioinformatics Basics in Next-Generation Sequencing
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Front Matter
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Next-generation Sequencing Methods, Platforms, Applications and Challenges
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Front Matter
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Next-generation Sequence Assembly Stages, Assessments, Tools and Challenges
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Front Matter
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Back Matter
About this book
The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field.
This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.
Authors and Affiliations
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Department of Computer Science, Mansoura University, Mansoura, Egypt
Sara El-Metwally
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Department of Information Technology, Mansoura University, Mansoura, Egypt
Osama M. Ouda
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Botany Department and Biotechnology Department, and The Donnelly Centre for Cellular and Biomolecular Research, Al-Azhar University, and University of Toronto (UofT), Cairo, Egypt
Mohamed Helmy
Bibliographic Information
Book Title: Next Generation Sequencing Technologies and Challenges in Sequence Assembly
Authors: Sara El-Metwally, Osama M. Ouda, Mohamed Helmy
Series Title: SpringerBriefs in Systems Biology
DOI: https://doi.org/10.1007/978-1-4939-0715-1
Publisher: Springer New York, NY
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: The Authors 2014
Softcover ISBN: 978-1-4939-0714-4Published: 19 April 2014
eBook ISBN: 978-1-4939-0715-1Published: 19 April 2014
Series ISSN: 2193-4746
Series E-ISSN: 2193-4754
Edition Number: 1
Number of Pages: XII, 118
Number of Illustrations: 11 b/w illustrations, 1 illustrations in colour
Topics: Systems Biology, Human Genetics, Bioinformatics