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New & Forthcoming Titles | The TNF Superfamily - Methods and Protocols

The TNF Superfamily

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1155

Bayry, Jagadeesh (Ed.)

2014, XI, 226 p. 43 illus., 15 illus. in color.

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  • Includes cutting-edge methods and protocols with a focus on TNF Superfamily members in health and disease
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Exploring these type II trans-membrane proteins, The TNF Superfamily: Methods and Protocols focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of investigating the role of TNF family members in physiopathologies, protocols on modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, as well as methodologies for various assays of TNF family members and the production of recombinant molecules.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and ready to use, The TNF Superfamily: Methods and Protocols will aid researchers investigating this key family of proteins, involved in vital processes such as providing signals for activation, differentiation, survival and death of cells, modulation of immune response and inflammation, hematopoiesis, and osteoclastogenesis.

Content Level » Professional/practitioner

Keywords » Cell signaling - Pathogenesis - Physiopathology - Recombinant molecules - TNF family member proteins - Type II trans-membrane proteins

Table of contents 

1. Assaying NF-kB Activation and Signaling from TNF Receptors

            Zhenzhen Wu and Leslie A. Bruggeman

2. Dissecting DR3 Signaling

            Yelena L. Pobezinskaya and Zhenggang Liu

3. Modulation of FceRI-Dependent Mast Cell Response by OX40L

            Riccardo Sibilano, Carlo Pucillo, and Barbara Frossi

4. Analyzing the Signaling Capabilities of Soluble and Membrane TWEAK

            Johannes Trebing, José Antonio Carmona Arana, Steffen Salzmann, and Harald Wajant

5. Regulation of Human Dendritic Cell Functions by Natural Anti-CD40 Antibodies

            Sri Ramulu Elluru, Srini V Kaveri, and Jagadeesh Bayry

 6. β-actin in the Signaling of Transmembrane TNF-α-Mediated Cytotoxicity

            Hui Chen, Yan Leng, and Zhuoya Li

7. Investigating the Protective Role of Death Receptor 3 (DR3) in Renal Injury Using an Organ Culture Model

            Rafia S. Al-Lamki

8. Analysis of TNF-Mediated Cerebral Pericyte Remodeling

            Jennifer V. Welser-Alves, Amin Boroujerdi, Ulrich Tigges, and Richard Milner 

9. CD137 in Chronic Graft-Versus-Host Disease

            Juyang Kim, Hogng R. Cho, and Byungsuk Kwon 

10. In Vitro Investigation of the Roles of the Proinflammatory Cytokines Tumor Necrosis Factor-α and Interleukin-1 in Murine Osteoclastogenesis

            Joel Jules and Xu Feng 

11. Evasion of TNF-α Mediated Apoptosis by Hepatitis C Virus

            Hangeun Kim and Ranjit Ray 

12. TNF-α Modulates TLR2-Dependent Responses During Mycobacterial Infection

            Sahana Holla, Jamma Trinath, and Kithiganahalli Narayanaswamy Balaji 

13. Experimental Applications of TNF-Reporter Mice with Far-Red Fluorescent Label

            Yury V. Shebzukhov, Anna A. Kuchmiy, Andrei A. Kruglov, Frauke Zipp, Volker Siffrin, and Sergei A. Nedospasov 

14. A Simple Solid-Phase Assay for Studying Direct Binding of Progranulin to TNFR and Progranulin Antagonism of TNF/TNFR Interactions

            Tian Qingyun, Shuai Zhao, and Chuanju Liu 

15. Compartment-Specific Flow Cytometry for the Analysis of TNF-Mediated Recruitment and Activation of Glomerular Leukocytes in Murine Kidneys

            Volker Vielhauer 

16. Determination of Soluble Tumor Necrosis Factor Receptor 2 Produced by Alternative Splicing

            Xavier Romero, Juan D. Cañete, and Pablo Engel 

17. Effective Expression and Purification of Bioactive Recombinant Soluble LIGHT

            Isamu Tsuji, Keiji Iwamoto, and Yasushi Shintani

18. One of the TNF Superfamily Members: Bi-Functional Protein, TNFR2-Fc-IL-1ra

            Bo Xie

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