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  • © 2013

Molecular Biophysics for the Life Sciences

  • Provides an overview of major research themes and research strategies in contemporary molecular biophysics
  • Introduces new investigators to major areas of biophysics
  • Explains the goals of biophysical research, while offering the tools available for investigation, the relevance of biological research to other fields, and future opportunities in the field

Part of the book series: Biophysics for the Life Sciences (BIOPHYS, volume 6)

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Table of contents (12 chapters)

  1. Front Matter

    Pages i-xii
  2. Introduction

    • Norma M. Allewell, Linda O. Narhi, Ivan Rayment
    Pages 1-15
  3. Structural, Physical, and Chemical Principles

    • Norma M. Allewell, Linda O. Narhi, Ivan Rayment
    Pages 17-30
  4. The Experimental Tools of Molecular Biophysics

    1. Front Matter

      Pages 31-31
    2. Optical Spectroscopic Methods for the Analysis of Biological Macromolecules

      • Linda O. Narhi, Cynthia H. Li, Ranjini Ramachander, Juraj Svitel, Yijia Jiang
      Pages 33-90
    3. Nuclear Magnetic Resonance Spectroscopy

      • Thomas C. Pochapsky, Susan Sondej Pochapsky
      Pages 113-173
    4. Electron Paramagnetic Resonance Spectroscopy

      • John H. Golbeck, Art van der Est
      Pages 175-213
    5. Mass Spectrometry

      • Igor A. Kaltashov, Cedric E. Bobst
      Pages 215-256
    6. Single-Molecule Methods

      • Paul J. Bujalowski, Michael Sherman, Andres F. Oberhauser
      Pages 257-288
  5. Biological Macromolecules as Molecular Machines: Three Examples

    1. Front Matter

      Pages 289-289
    2. Helicase Unwinding at the Replication Fork

      • Divya Nandakumar, Smita S. Patel
      Pages 291-312
    3. Rotary Motor ATPases

      • Stephan Wilkens
      Pages 313-339
    4. Biophysical Approaches to Understanding the Action of Myosin as a Molecular Machine

      • Mihály Kovács, András Málnási-Csizmadia
      Pages 341-361
  6. Future Prospects

    1. Front Matter

      Pages 363-363
    2. Future Prospects

      • Norma M. Allewell, Igor A. Kaltashov, Linda O. Narhi, Ivan Rayment
      Pages 365-379
  7. Back Matter

    Pages 381-397

About this book

This volume provides an overview of the development and scope of molecular biophysics and in-depth discussions of the major experimental methods that enable biological macromolecules to be studied at atomic resolution.   It also reviews the physical chemical concepts that are needed to interpret the experimental results and to understand how the structure, dynamics, and physical properties of biological macromolecules enable them to perform their biological functions.  Reviews of research on three disparate biomolecular machines—DNA helicases, ATP synthases, and myosin--illustrate how the combination of theory and experiment leads to new insights and new questions. 

Reviews

From the reviews:

“This valuable work describes the major methods used for characterizing biological macromolecules. The book is very readable and well organized, with 12 succinct chapters. … Summing Up: Highly recommended. Upper-division undergraduates and above.” (J. A. Kelly, Choice, Vol. 51 (10), June, 2014)

Editors and Affiliations

  • Chemistry and Biochemistry, University of Maryland, College Park, USA

    Norma Allewell

  • Research & Development, Amgen, Inc., Thousand Oaks, USA

    Linda O. Narhi

  • Department of Biochemistry, University of Wisconsin - Madison, Madison, USA

    Ivan Rayment

About the editors

Dr. Norma M. Allewell is Professor of Cell Biology and Molecular Genetics and Affiliate Professor of Chemistry and Biochemistry at the University of Maryland, where she served as Dean of the College of Chemical and Life Sciences for a decade. Her research focuses on protein structure, function and dynamics, and metabolic regulatory mechanisms and diseases.

Dr. Linda Narhi is a Scientific Executive Director in the Product Attribute Science Group at Amgen, where her responsibilities include solution stability assessment of all protein-based therapeutic candidates, and developing and implementing predictive assays for protein stability to process, storage, and delivery conditions.

Dr. Ivan Rayment is Professor of Biochemistry at the University of Wisconsin-Madison, where he holds the Michael G. Rossmann Professorship in Biochemistry. He has a wide range of interests in structural biology and has made seminal contributions to our understanding of the structural basis of motility, enzyme evolution, cobalamin biosynthesis, and transposition.

Bibliographic Information

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access