Molecular Diversity and PCR-detection of Toxigenic Fusarium Species and Ochratoxigenic Fungi

1. Front Matter

   Pages i-vii

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2. Phylogeny and molecular diagnosis of mycotoxigenic fungi

   • Keith A. Seifert, C. André Lévesque

   Pages 449-471

3. FUSARIUM-ID v. 1.0: A DNA sequence database for identifying Fusarium

   • David M. Geiser, María del Mar Jiménez-Gasco, Seogchan Kang, Izabela Makalowska, Narayanan Veeraraghavan, Todd J. Ward et al.

   Pages 473-479

4. Quantitative detection of Fusarium species in wheat using TaqMan

   • Cees Waalwijk, Ruth van der Heide, Ineke de Vries, Theo van der Lee, Cor Schoen, Guillaume Costrel-de Corainville et al.

   Pages 481-494

5. A species-specific PCR assay based on the calmodulin partial gene for identification of Fusarium verticillioides, F. proliferatum and F. subglutinans

   • G. Mulè, A. Susca, G. Stea, A. Moretti

   Pages 495-502

6. Detection and differentiation of trichothecene and enniatin-producing Fusarium species on small-grain cereals

   • P. Nicholson, D. R. Simpson, A. H. Wilson, E. Chandler, M. Thomsett

   Pages 503-514

7. Differentiation of Fusarium verticillioides from banana fruits by IGS and EF-1α sequence analyses

   • S. Mirete, C. Vázquez, G. Mulè, M. Jurado, M. T. González-Jaén

   Pages 515-523

8. Genetic markers for the analysis of variability and for production of specific diagnostic sequences in fumonisin-producing strains of Fusarium verticillioides

   • M. Teresa González-Jaén, Salvador Mirete, Belén Patiño, Elena López-Errasquín, Covadonga Vázquez

   Pages 525-532

9. Synteny in toxigenic Fusarium species: The fumonisin gene cluster and the mating type region as examples

   • Cees Waalwijk, Theo van der Lee, Ineke de Vries, Thamara Hesselink, Joop Arts, Gert H. J. Kema

   Pages 533-544

10. Mitochondrial DNA diversity and lineage determination of European isolates of Fusarium graminearum (Gibberella zeae)

    • M. Láday, Á. Juhász, G. Mulè, A. Moretti, Á. Szécsi, A. Logrieco

    Pages 545-550

11. Genetic diversity of Fusarium graminearum in Europe and Asia

    • T. Yu. Gagkaeva, T. Yli-Mattila

    Pages 551-562

12. Mitochondrial DNA variability in Fusarium proliferatum (Gibberella intermedia)

    • M. Láday, G. Mulè, A. Moretti, Z. Hamari, Á. Juhász, Á. Szécsi et al.

    Pages 563-571

13. Molecular and morphological diversity of Fusarium species in Finland and northwestern Russia

    • T. Yli-Mattila, S. Paavanen-Huhtala, P. Parikka, P. Konstantinova, T. Y. Gagkaeva

    Pages 573-585

14. Mycotoxin production and molecular variability of European and American isolates of Fusarium culmorum

    • Beáta Tóth, Ákos Mesterházy, Paul Nicholson, József Téren, János Varga

    Pages 587-599

15. Toxin profile, fertility and AFLP analysis of Fusarium verticillioides from banana fruits

    • A. Moretti, G. Mulè, A. Susca, M. T. González-Jaén, A. Logrieco

    Pages 601-609

16. Interfertility of two mating populations in the Gibberella fujikuroi species complex

    • John F. Leslie, Kurt A. Zeller, Melissa Wohler, Brett A. Summerell

    Pages 611-618

17. Identification of growth stage specific transcript profiles in Fusarium proliferatum (Gibberella fujikuroi, mating population D) by cDNA-AFLP analysis

    • A. Jeney, E. Béki, G. Mulè, L. Hornok

    Pages 619-625

18. Molecular diversity of agriculturally important Aspergillus species

    • János Varga, Ákos Juhász, Ferenc Kevei, Zofia Kozakiewicz

    Pages 627-640

19. PCR assay for identification of Aspergillus carbonarius and Aspergillus japonicus

    • G. Perrone, A. Susca, G. Stea, G. Mulè

    Pages 641-649

20. Production of ochratoxin A by Aspergillus ochraceus

    • Ellen Mühlencoert, Ines Mayer, Michael W. Zapf, Rudi F. Vogel, Ludwig Niessen

    Pages 651-659

Toxigenic Fusarium species and ochratoxigenic fungi are responsible for various plant diseases which have important consequential effects on both human and animal health worldwide. The development of rapid, robust and sensitive detection methods based on new molecular technologies is urgently needed in order to identify fungal contamination in the field and quantify toxin accumulation in food and animal feed. Most of the contributions in this special issue are from results obtained through the EU 5th Framework project (QLKI-CT-1998-01380) "DETOX-FUNGI: early detection of toxigenic Fusarium species and ochratoxi­ genic fungi in plant products", which has strongly stimulated interaction and co-operation between many European scientists. Valuable contributions from other scientists have guaranteed a complete overview of this stimulating and interesting topic. This is the third special issue published in the European Journal of Plant Pathology concerning my­ cotoxigenic fungi under the aegis of COST Action 835 'Agriculturally Important Toxigenic Fungi'. The first two dealt with 'Mycotoxins in Plant Disease' (Vol. 108(7) 2002) and 'Epidemiology of Mycotoxin Pro­ ducing Fungi' (Vol. 109(7) 2003). The present issue contains contributions which cover aspects of molecular diversity, phylogeny and PCR-detection of toxigenic Fusarium species and various ochratoxigenic fungi. We hope these will prove helpful to researchers involved in similar work and will stimulate the future studies required for the early detection of these fungi, which is so essential for overcoming the health risks associated with mycotoxin-contaminated food products.

• Aspergillus
• DNA
• Fungi
• Toxin
• cereals
• phylogeny
• wheat

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