Editors:
- Encompasses lipoprotein-centered methodologies designed for all experience levels
- Features detailed protocols that are lab-ready
- Includes expert tips and key implementation advice to ensure successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 1027)
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Table of contents (18 protocols)
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Front Matter
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RNA and Gene Expression
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Front Matter
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Transgenic, Knockout, and Knockdown Methodologies
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Front Matter
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Special Topics
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Front Matter
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About this book
The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies. In Lipoproteins and Cardiovascular Disease: Methods and Protocols, experts in the field present a compendium of advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and vascular biology research, bringing together in a single volume an updated set of protocols and strategies for methods now driving the most recent advances, along with classical methods that are still widely used. Among the many topics covered in this cutting-edge work, the book delves into crucial techniques such as quantitative real-time PCR, microarrays, RT-PCR laser capture microdissection, and tissue-specific gene overexpression, knockout, and knockdown methodologies, including AAV as a liver-directed gene delivery vehicle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable notes which highlight tips on troubleshooting and avoiding known pitfalls.
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Comprehensive and easy to use, Lipoproteins and Cardiovascular Disease: Methods and Protocols serves both novices and experts alike as a complete guide for any researcher with an interest in lipoproteins and their significant biological effects.Editors and Affiliations
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Pulmonary & Vascular Medicine Branch, National Institutes of Health (NIH) National Heart, Lung & Blood Institute, Bethesda, USA
Lita A. Freeman
Bibliographic Information
Book Title: Lipoproteins and Cardiovascular Disease
Book Subtitle: Methods and Protocols
Editors: Lita A. Freeman
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-369-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-60327-368-8
Softcover ISBN: 978-1-4939-5690-6
eBook ISBN: 978-1-60327-369-5
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 391
Number of Illustrations: 27 b/w illustrations, 23 illustrations in colour
Topics: Protein Science, Cardiology