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Fluorescence Spectroscopy and Microscopy

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Yves Engelborghs

    1. KULeuven-Biomolecular Dynamics BioSCENTer, Leuven, Belgium

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    You can also search for this editor in PubMed   Google Scholar

  2. Antonie J.W.G. Visser

    1. MicroSpectroscopy Centre, University of Wageningen Biochemistry, Wageningen, The Netherlands

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Explores the methods and applications in the expanding fluorescence field
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1076)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xxi
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  2. Steady-State Fluorescence Spectroscopy (SSFS)

    1. Front Matter

      Pages 1-1
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    2. How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra

      • Adam Matthew Gilmore
      Pages 3-27

    3. Steady-State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions

      • Nicholas G. James, David M. Jameson
      Pages 29-42

    4. Quantitative Fluorescence Spectral Analysis of Protein Denaturation

      • Ivo H. M. van Stokkum, Sergey P. Laptenok
      Pages 43-51

    5. High-Pressure Fluorescence Applications

      • Mariano Dellarole, Catherine A. Royer
      Pages 53-74

  3. Time-Resolved Fluorescence Spectroscopy (TRFS)

    1. Front Matter

      Pages 75-75
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    2. Frequency Domain Fluorometry: Theory and Application

      • Carissa M. Vetromile, David M. Jameson
      Pages 77-95

    3. Polar Plot Representation of Time-Resolved Fluorescence

      • John Paul Eichorst, Kai Wen Teng, Robert M. Clegg
      Pages 97-112

    4. Ensemble and Single-Molecule Detected Time-Resolved FRET Methods in Studies of Protein Conformations and Dynamics

      • Tomer Orevi, Eitan Lerner, Gil Rahamim, Dan Amir, Elisha Haas
      Pages 113-169

    5. MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes

      • Patrik R. Callis, Jose R. Tusell
      Pages 171-214

    6. Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins

      • Dmitri Toptygin
      Pages 215-256

    7. Global Analysis of Time-Resolved Fluorescence Data

      • Anatoli V. Digris, Eugene G. Novikov, Victor V. Skakun, Vladimir V. Apanasovich
      Pages 257-277

    8. Nanometrology

      • David J. S. Birch, Philip Yip
      Pages 279-302

    9. Upconversion Spectrophotofluorometry

      • Arianna Biesso, Jianhua Xu, Jay R. Knutson
      Pages 303-319

    10. Subpicosecond Kerr-Gate Spectrofluorometry

      • Sergey P. Laptenok, Patrick Nuernberger, Andras Lukacs, Marten H. Vos
      Pages 321-336

    11. Photoinduced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay

      • Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, Fumio Tanaka
      Pages 337-355

  4. Fluorescent Probe Development (FPD)

    1. Front Matter

      Pages 357-357
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    2. Biosynthetic Incorporation of Tryptophan Analogs in Proteins

      • Jaap Broos
      Pages 359-370

    3. Optimization of Fluorescent Proteins

      • Daphne S. Bindels, Joachim Goedhart, Mark A. Hink, Laura van Weeren, Linda Joosen, Theodorus W. J. Gadella Jr.
      Pages 371-417
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Keywords

About this book

Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.  The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).  Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.

 

Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

Reviews

From the reviews:

“The book is illustrated with high-quality figures in both color and black and white. … This book offers techniques from internationally recognized experts. Both novice and established scientists will find it extremely useful.” (Omer Iqbal, Doody’s Book Reviews, March, 2014)

Editors and Affiliations

  • KULeuven-Biomolecular Dynamics BioSCENTer, Leuven, Belgium

    Yves Engelborghs

  • MicroSpectroscopy Centre, University of Wageningen Biochemistry, Wageningen, The Netherlands

    Antonie J.W.G. Visser

Bibliographic Information

  • Book Title: Fluorescence Spectroscopy and Microscopy

  • Book Subtitle: Methods and Protocols

  • Editors: Yves Engelborghs, Antonie J.W.G. Visser

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-649-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-648-1Published: 10 October 2013

  • Softcover ISBN: 978-1-4939-6315-7Published: 23 August 2016

  • eBook ISBN: 978-1-62703-649-8Published: 10 October 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXI, 816

  • Number of Illustrations: 73 b/w illustrations, 171 illustrations in colour

  • Topics: Imaging / Radiology, Biological Microscopy, Spectroscopy/Spectrometry

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