Skip to main content
SpringerLink
Log in

Atomic Force Microscopy in Biomedical Research

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Pier Carlo Braga

    1. School of Medicine, Department of Pharmacology, University of Milan, Milano, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Davide Ricci

    1. , Robotics, Brain & Cognitive Sciences, Italian Institute of Technology, Genova, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Applicable to a wide variety of fields and disciplines
  • Features a comprehensive collection of practical applications and techniques
  • Includes tips from the experts as well as key implementation advice
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 736)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 179.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. The Basics of Atomic Force Microscopy

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. How the Atomic Force Microscope Works?

      • Bruno Torre, Davide Ricci, Pier Carlo Braga
      Pages 3-18

    3. Measurement Methods in Atomic Force Microscopy

      • Bruno Torre, Claudio Canale, Davide Ricci, Pier Carlo Braga
      Pages 19-29

    4. Recognizing and Avoiding Artifacts in Atomic Force Microscopy Imaging

      • Claudio Canale, Bruno Torre, Davide Ricci, Pier Carlo Braga
      Pages 31-43

  3. Molecule Imaging

    1. Front Matter

      Pages 45-45
      Download chapter PDF

    2. Imaging the Spatial Orientation of Subunits Within Membrane Receptors by Atomic Force Microscopy

      • Stewart M. Carnally, J. Michael Edwardson, Nelson P. Barrera
      Pages 47-60

    3. High Resolution Imaging of Immunoglobulin G Antibodies and Other Biomolecules Using Amplitude Modulation Atomic Force Microscopy in Air

      • Sergio Santos, Neil H. Thomson
      Pages 61-79

    4. Atomic Force Microscopy of Ex Vivo Amyloid Fibrils

      • Claudio Canale, Annalisa Relini, Alessandra Gliozzi
      Pages 81-95

    5. Studying Collagen Self-Assembly by Time-Lapse High-Resolution Atomic Force Microscopy

      • Clemens M. Franz, Daniel J. Muller
      Pages 97-107

    6. Atomic Force Microscopy Imaging of Human Metaphase Chromosomes in Liquid

      • Osamu Hoshi, Tatsuo Ushiki
      Pages 109-115

    7. Atomic Force Microscopy of Proteasome Assemblies

      • Maria Gaczynska, Pawel A. Osmulski
      Pages 117-132

    8. Atomic Force Microscopy of Isolated Mitochondria

      • Bradley E. Layton, M. Brent Boyd
      Pages 133-151

    9. Imaging and Interrogating Native Membrane Proteins Using the Atomic Force Microscope

      • Andreas Engel
      Pages 153-167

  4. Nanoscale Surface Analysis and Cell Imaging

    1. Front Matter

      Pages 169-169
      Download chapter PDF

    2. Atomic Force Microscopy Investigation of Viruses

      • Alexander McPherson, Yurii G. Kuznetsov
      Pages 171-195

    3. Determination of the Kinetic On- and Off-Rate of Single Virus–Cell Interactions

      • Christian Rankl, Linda Wildling, Isabel Neundlinger, Ferry Kienberger, Hermann Gruber, Dieter Blaas et al.
      Pages 197-210

    4. Atomic Force Microscopy as a Tool for the Study of the Ultrastructure of Trypanosomatid Parasites

      • Wanderley de Souza, Gustavo M. Rocha, Kildare Miranda, Paulo M. Bisch, Gilberto Weissmuller
      Pages 211-221

    5. Normal and Pathological Erythrocytes Studied by Atomic Force Microscopy

      • Andreas Ebner, Hermann Schillers, Peter Hinterdorfer
      Pages 223-241

    6. The Growth Cones of Living Neurons Probed by the Atomic Force Microscope

      • Davide Ricci, Massimo Grattarola, Mariateresa Tedesco
      Pages 243-257
Back to top

Keywords

About this book

With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life.  Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use.  Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.

Editors and Affiliations

  • School of Medicine, Department of Pharmacology, University of Milan, Milano, Italy

    Pier Carlo Braga

  • , Robotics, Brain & Cognitive Sciences, Italian Institute of Technology, Genova, Italy

    Davide Ricci

Bibliographic Information

  • Book Title: Atomic Force Microscopy in Biomedical Research

  • Book Subtitle: Methods and Protocols

  • Editors: Pier Carlo Braga, Davide Ricci

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-105-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-104-8Published: 20 May 2011

  • Softcover ISBN: 978-1-4939-6279-2Published: 23 August 2016

  • eBook ISBN: 978-1-61779-105-5Published: 10 June 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 508

  • Number of Illustrations: 212 b/w illustrations, 5 illustrations in colour

  • Topics: Imaging / Radiology, Biological Microscopy

Publish with us

Policies and ethics

Back to top

Search

Navigation