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Biosensors and Biodetection

Methods and Protocols Volume 2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors

  • Unique, one-of-a-kind laboratory manual for detailed, standardized protocols on biosensors or lab-on-a-chip technology
  • Describes basic types and the basic elements of biosensors from an experimental point of view
  • Provides multiple experimental approaches to enable readers to choose an appropriate design and to combine elements from different designs
  • Includes enough technical information to promote understanding of the technology and the ability to conduct similar experiments

Part of the book series: Methods in Molecular Biology (MIMB, volume 504)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xviii
  2. Mechanical Detectors

    1. A Set of Piezoelectric Biosensors Using Cholinesterases

      • Carsten Teller, Jan Halámek, Alexander Makower, Frieder W. Scheller
      Pages 3-22
    2. Piezoelectric Biosensors for Aptamer—Protein Interaction

      • Sara Tombelli, Alessandra Bini, Maria Minunni, Marco Mascini
      Pages 23-36
    3. Biosensors Based on Cantilevers

      • Mar Álvarez, Laura G. Carrascosa, Kiril Zinoviev, Jose A. Plaza, Laura M. Lechuga
      Pages 51-71
  3. Electrochemical Detectors

    1. Electrochemical Biosensor Technology: Application to Pesticide Detection

      • Ilaria Palchetti, Serena Laschi, Marco Mascini
      Pages 115-126
    2. Electrochemical Detection of DNA Hybridization Using Micro and Nanoparticles

      • María Teresa Castañeda, Salvador Alegret, Arben Merkoçi
      Pages 127-143
    3. Electrochemical Immunosensing Using Micro and Nanoparticles

      • Alfredo de la Escosura-Muñiz, Adriano Ambrosi, Salvador Alegret, Arben Merkoçi
      Pages 145-155
    4. Methods for the Preparation of Electrochemical Composite Biosensors Based on Gold Nanoparticles

      • A. González-Cortés, P. Yáñez-Sedeño, J. M. Pingarrón
      Pages 157-166
  4. Lateral Flow

    1. Immunochromatographic Lateral Flow Strip Tests

      • Gaiping Zhang, Junqing Guo, Xuannian Wang
      Pages 169-183
    2. Rapid Prototyping of Lateral Flow Assays

      • Alexander Volkov, Michael Mauk, Paul Corstjens, R. Sam Niedbala
      Pages 217-235
    3. Lateral Flow Colloidal Gold-Based Immunoassay for Pesticide

      • Shuo Wang, Can Zhang, Yan Zhang
      Pages 237-252
  5. Ligands

    1. Synthesis of a Virus Electrode for Measurement of Prostate Specific Membrane Antigen

      • Juan E. Diaz, Li-Mei C. Yang, Jorge A. Lamboy, Reginald M. Penner, Gregory A. Weiss
      Pages 255-274
    2. Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands

      • Michael J. McGuire, Shunzi Li, Kathlynn C. Brown
      Pages 291-321
    3. Biosensor Detection Systems: Engineering Stable, High-Affinity Bioreceptors by Yeast Surface Display

      • Sarah A. Richman, David M. Kranz, Jennifer D. Stone
      Pages 323-350

About this book

Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors focuses on direct measurement sensors, indirect methods, ligands, and related technologies, including methods involving electrochemical detectors, recognition ligands, antibodies, aptamers, and peptides, amongst many other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.

Editors and Affiliations

  • FDA Center for Devices and Radiological Health, Silver Spring, USA

    Avraham Rasooly

  • National Cancer Institute, Bethesda, USA

    Avraham Rasooly

  • Fischell Department of Bioengineering, University of Maryland, College Park, USA

    Keith E. Herold

Bibliographic Information

  • Book Title: Biosensors and Biodetection

  • Book Subtitle: Methods and Protocols Volume 2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors

  • Editors: Avraham Rasooly, Keith E. Herold

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-569-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60327-568-2Published: 12 December 2008

  • Softcover ISBN: 978-1-61737-912-3Published: 19 November 2010

  • eBook ISBN: 978-1-60327-569-9Published: 22 January 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 464

  • Topics: Biotechnology, Imaging / Radiology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access