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Medicine - Pathology | Modern Clinical Molecular Techniques

Modern Clinical Molecular Techniques

Hu, Peter, Hegde, Madhuri, Lennon, Patrick Alan (Eds.)

2012, XIII, 436p. 60 illus., 41 illus. in color.

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  • Presents the essentials of daily laboratory practice
  • Covers oncology testing, pre/post-natal testing, infectious disease testing, and identity testing
  • Details the most up-to-date protocols in molecular pathology
  • Written by the world’s foremost authorities in clinical molecular laboratory science

This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today’s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure.  It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice.

Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.

Content Level » Professional/practitioner

Keywords » Amplification - DNA - Electrophoresis - Genetics - Genotype

Related subjects » Internal Medicine - Molecular Medicine - Oncology & Hematology - Pathology - Public Health

Table of contents 






Section I   Current Molecular Laboratory Methods


1        Specimen Collection, Handling and Processing

Lindsy Hengesbach and John Gerlach


2        DNA/RNA Isolation and Quantitation

Jing Wang


3        Test Validation

Cindy McCloskey and S Terence Dunn


4        In Vitro Amplification Methods in Molecular Diagnostics

C. Alexander Valencia and Bradford Coffee


5        Whole Genome Sequencing in the Clinical Laboratory

Tina Hambuch, Brad Sickler, Arnold Liao, Suneer Jain, and Philip D. Cotter


6        Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction

Juliana Purrazzella and Arupa Ganguly


7        Reporting Clinical Molecular Genetic Laboratory Results

Victor Wei Zhang and Lora J. H. Bean



Section II Clinical Molecular Infectious Disease Testing


8        HIV-1 Genotyping and Virtual Phenotyping

Fangling Xu and Jianli Dong


9        COBAS® Ampliprep/Taqman HIV-1 Test

Tavat A. Buraruk and Charles E. Stager


10    Hepatitis C Virus (HCV) Genotype Assay (LiPA)

Charles E. Stager and Tavat A. Buraruk


11    COBAS® Ampliprep/Taqman HCV Test

Charles E. Stager and Tavat A. Buraruk


12    Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction

Su S. Chen, Seema Hai, and Rajyalakshmi Luthra


13    Quantification of BK Viral Load

Hai Wu and Jianli Dong


14    Molecular Diagnosis and Epidemiology of Rabies

Rodney E. Rohde and Bonny C. Mayes


15    Pyrosequencing for Bacterial Identification

Laura Millecker and Ruth Ann Luna


16    Other Pathogens of Significant Public Health Concern

Chris L. McGowin, Rodney E. Rohde, and Greg C. Whitlock




Section III Clinical Molecular Oncology Testing


17    Gene Rearrangements Testing

Siby Sebastian, Juan Du, and Rizwan Naeem


18    Mutation Analysis in Cancer

Keyur Pravinchandra Patel, John Galbincea,  and Rajyalakshmi Luthra


19    Minimal Residual Disease

Wesley Greaves and Rajyalakshmi Luthra


20    Pyrosequencing in Cancer

Bedia A. Barkoh, Rajyalakshmi Luthra, and Shalini Verma


21    Clinical Applications of Microarrays in Cancer

Marilyn M. Li, Ankita Patel, and Xiaofeng Hu


22    Basic Theories and Clinical Applications of Molecular Flow Cytometry

Hua Kang Wu, Hongli Chen, and Peter Hu



Section IV Clinical Molecular Pre/Postnatal Testing


23    Molecular Prenatal Diagnostic Testing

Joanna Wiszniewska and Christine M. Eng


24    PCR Based Diagnosis of Fragile X Syndrome 

Daniel J. Dykas and Allen E. Bale



Section V Human Identity Testing


25    Microsatellite Testing Short Tandem Repeat Profile Generation

Robin DeVille Guidry and Laura Gahn


26    Parentage

Laura Gahn and Robin DeVille Guidry



Section VI Clinical Molecular Training


27    Diagnostic Molecular Training

Peter Hu, Patrick A. Lennon, and Madhuri Hegde


28    Molecular Diagnostics Point of Care Testing Training

Chang W. Lee, and Peter Hu


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