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Medicine - Oncology & Hematology | Cancer Stem Cells - Methods and Protocols

Cancer Stem Cells

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 568

Yu, John S. (Ed.)

2009

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  • Provides easy to follow protocols in this technically challenging field
  • Presents a cohesive review of a variety of methods to isolate, identify, and study cancer stem cells
  • A timely contribution in this nascent field, the book will level the playing field of investigators of cancer stem cells
  • Presents a comprehensive, step-by-step guide by leading researchers in the field of cancer stem cells
  • Brings to light methods that have been mired in mystery
  • Brings together divergent viewpoints and methods to isolate the same population of cells, from prospective isolation using surface markers to stem cell propagation assays

Through the revolutionary concept of cancer stem cells, cancer research has been reinvigorated to study the role of these unique cells in cancer propagation and as targets of innovative therapies. In Cancer Stem Cells: Methods and Protocols, preeminent researchers have compiled cancer stem cell research techniques and protocols to promote healthy competition, discourse, and collaboration in this vital field.  The volume covers extensive topics such as identification and isolation of cancer stem cells, animal models of cancer stem cells, methylation profiling, the contribution of the niche in the regulation of cancer stem cells, immunologic targeting, and the use of normal stem cells as a treatment, among other subjects.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Cancer Stem Cells: Methods and Protocols will aid scientists around the world in the furthering of our understanding of cancer initiation and propagation and, most importantly, in the development of novel targets for cancer therapy.

Content Level » Research

Keywords » Brain cancer - Carcinom - DNA - Head and neck cancer - Leukemia - Marker expression - Neurospheres - Pancreatic cancer - Pituitary adenoma - Prostate cancer - Tumors - molecular biology

Related subjects » Cancer Research - Cell Biology - Life Sciences - Oncology & Hematology

Table of contents 

1. Defining Cancer Stem Cells by Xenotransplantation in Zebrafish Michael C. Dovey and Leonard I. Zon 2. Analysis of Cancer Stem Cell Metastasis in Xenograft Animal Models Yibin Kang 3. Identification of Murine and Human Acute Myeloid Leukemia Stem Cells Aniruddha J. Deshpande, Farid Ahmed, and Christian Buske 4. Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal Ichiro Nakano and Harley I. Kornblum 5. Prospective Identification of Cancer Stem Cells with the Surface Antigen CD133 Kyeung Min Joo and Do-Hyun Nam 6. Neurosphere Culture and Human Organotypic Model to Evaluate Brain Tumor Stem Cells Hugo Guerrero-Cázares, Kaisorn L. Chaichana, and Alfredo Quiñones-Hinojosa 7. Methodologies in Assaying Prostate Cancer Stem Cells Hangwen Li, Ming Jiang, Sofia Honorio, Lubna Patrawala, Collene R. Jeter, Tammy Calhoun-Davis, Simon W. Hayward, and Dean G. Tang 8. Characterization of Non-Malignant and Malignant Prostatic Stem/Progenitor Cells by Hoechst Side Population Method Murielle Mimeault and Surinder K. Batra 9. Anchorage Independent Growth of Prostate Cancer Stem Cells Shunyou Wang 10. Identification of Human Pancreatic Cancer Stem Cells Chenwei Li, C.J. Lee, and Diane M. Simeone 11. Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma Laurie Ailles and Mark Prince 12. Pituitary Adenoma Stem Cells Patrizia Tunici and John S. Yu 13. Genome-Wide DNA Methylation Profiling: The mDIP-Chip Technology Yin Shen, Shaun D. Fouse, and Guoping Fan 14. The Contribution of Niche-Derived Factors to the Regulation of Cancer Cells Julie B. Sneddon 15. Dendritic Cell Vaccines for Cancer Stem Cells Serena Pellegatta and GaetanoFinocchiaro 16. Maintaining and Engineering Neural Stem Cells for Delivery of Genetically Encoded Therapy to Brain Tumors Jennifer Katz, Joseph Krueger, Brunhilde Felding-Habermann, and Evan Y. Snyder 17. Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cells Vera S. Donnenberg, E. Michael Meyer, and Albert D. Donnenberg

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