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  • Book
  • © 2003

Tumor Suppressor Genes

Volume 2: Regulation, Function, and Medicinal Applications

Editors:

  1. Wafik S. El-Deiry

    1. Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia
      Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia
      Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 223)

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Table of contents (39 protocols)

  1. Front Matter

    Pages i-xix
    PDF

  2. Understanding the Function and Regulation of Tumor Suppressor Genes

    1. Front Matter

      Pages 1-1
      PDF

    3. Generation and Application of Phospho-specific Antibodies for p53 and pRB

      • Yoichi Taya, Kiichiro Nakajima, Kumiko Yoshizawa-Kumagaye, Katsuyuki Tamai
      Pages 17-26

    4. Stability and Ubiquitination of the Tumor Suppressor Protein p53

      • Lesley J. Jardine, Carl G. Maki
      Pages 27-38

    5. Role of Tumor Suppressors in DNA Damage Response

      • Bin-Bing S. Zhou, Michael R. Mattern, Kum Kum Khanna
      Pages 39-50

    6. In Situ Hybridization in Cancer and Normal Tissue

      • Shrihari Kadkol, Jeremy Juang, Tzyy-choou Wu
      Pages 51-72

    7. Genetic Strategies in Saccharomyces cerevisiae to Study Human Tumor Suppressor Genes

      • Takahiko Kobayashi, Ting Wang, Hua Qian, Rainer K. Brachmann
      Pages 73-86

    8. Electrophoretic Mobility Shift Analysis of the DNA Binding of Tumor Suppressor Gene Products

      • Edward C. Thornborrow, Matthew Maurer, James J. Manfredi
      Pages 87-100

    9. Analysis of Gene Promoter Regulation by Tumor Suppressor Genes

      • Kumaravel Somasundaram, Sanjeev Das, Smita Lakhotia, Narendra Wajapeyee
      Pages 101-116

    10. DNA Footprinting

      • M. Stacey Ricci, Wafik S. El-Deiry
      Pages 117-127

    13. Microarray Approaches for Analysis of Tumor Suppressor Gene Function

      • Sally A. Amundson, Albert J. Fornace Jr.
      Pages 141-153

    15. Yeast Two-Hybrid Screening as a Means of Deciphering Tumor Suppressor Pathways

      • E. Robert McDonald III, Wafik S. El-Deiry
      Pages 173-185

    16. Somatic Cell Knockouts of Tumor Suppressor Genes

      • Ruth Hemmer, Wenyi Wei, Annie Dutriaux, John M. Sedivy
      Pages 187-206

    18. Flow Cytometric Analysis of Cell Cycle Control by Tumor Suppressor Genes

      • David T. Dicker, Wafik S. El-Deiry
      Pages 211-215

    19. Analysis of Cyclin-Dependent Kinase Activity

      • Timothy K. MacLachlan, Wafik S. El-Deiry
      Pages 217-220
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About this book

It has become clear that tumors result from excessive cell proliferation and a corresponding reduction in cell death caused by the successive accumulation of mutations in key regulatory target genes over time. During the 1980s, a number of oncogenes were characterized, whereas from the 1990s to the present, the emp- sis has shifted to tumor suppressor genes (TSGs). It has become clear that oncogenes and TSGs function in the same pathways, providing positive and negative growth regulatory activities. The signaling pathways controlled by these genes involve virtually every process in cell biology, including nuclear events, cell cycle, cell death, cytoskeletal, cell membrane, angiogenesis, and cell adhesion effects. Mu- tions in tumor suppressor genes have been identified in familial cancer syndromes, and the same genes in many cases have been found to be mutationally inactivated in sporadically occurring cancers. In their normal state, TSGs control cancer development and progression, as well as contribute to the sensitivity of cancers to a variety of therapeutics. Understanding the classes of TSGs, the biochemical pa- ways they function in, and how they are regulated provides an essential lesson in cancer biology. We cannot hope to advance our current knowledge and to develop new and more effective therapies without understanding the relevant pathways and how they influence the present approaches to therapy. Moreover, it is important to be able to access not only the powerful tools now available to discover these genes, but also their links to cell biology and growth control.
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Reviews

"The book will prove a great help to everybody who would like to enter the field of experimental and or clinical research. Those who are interested in basic understanding microbiology of cancer will enjoy this as well." -Neoplasma
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Editors and Affiliations

  • Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia

    Wafik S. El-Deiry

  • Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia

    Wafik S. El-Deiry

  • Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia

    Wafik S. El-Deiry

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Bibliographic Information

  • Book Title: Tumor Suppressor Genes

  • Book Subtitle: Volume 2: Regulation, Function, and Medicinal Applications

  • Editors: Wafik S. El-Deiry

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592593291

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2003

  • Hardcover ISBN: 978-0-89603-987-2Published: 21 April 2003

  • Softcover ISBN: 978-1-4899-3914-2Published: 09 October 2013

  • eBook ISBN: 978-1-59259-329-3Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIX, 657

  • Topics: Oncology

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Buy it now

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

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