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  • © 2014

Staphylococcus Epidermidis

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1106)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
  2. Pulsed Field Gel Electrophoresis of Staphylococcus epidermidis

    • Richard V. Goering, Paul D. Fey
    Pages 55-60
  3. Multilocus Sequence Typing of Staphylococcus epidermidis

    • Jonathan C. Thomas, D. Ashley Robinson
    Pages 61-69
  4. Genetic Manipulation of Staphylococci

    • Jeffrey L. Bose
    Pages 101-111
  5. Isolation of Staphylococcus sp. RNA

    • Tess Eidem
    Pages 119-124
  6. Methods to Generate a Sequence-Defined Transposon Mutant Library in Staphylococcus epidermidis Strain 1457

    • Todd J. Widhelm, Vijay Kumar Yajjala, Jennifer L. Endres, Paul D. Fey, Kenneth W. Bayles
    Pages 135-142
  7. Examination of Staphylococcus epidermidis Biofilms Using Flow-Cell Technology

    • Derek E. Moormeier, Kenneth W. Bayles
    Pages 143-155
  8. Rapid Quantitative and Qualitative Analysis of Biofilm Production by Staphylococcus epidermidis Under Static Growth Conditions

    • Elaine M. Waters, Hannah McCarthy, Siobhan Hogan, Marta Zapotoczna, Eoghan O’Neill, James P. O’Gara
    Pages 157-166
  9. Bacteriophage Transduction in Staphylococcus epidermidis

    • Michael E. Olson, Alexander R. Horswill
    Pages 167-172
  10. Mouse Model of Post-arthroplasty Staphylococcus epidermidis Joint Infection

    • Tyler D. Scherr, Kevin E. Lindgren, Carolyn R. Schaeffer, Mark L. Hanke, Curtis W. Hartman, Tammy Kielian
    Pages 173-181
  11. A Mouse Model of Staphylococcus Catheter-Associated Biofilm Infection

    • Cortney E. Heim, Mark L. Hanke, Tammy Kielian
    Pages 183-191
  12. Rat Jugular Catheter Model of Biofilm-Mediated Infection

    • Carolyn R. Schaeffer, Keith M. Woods, G. Matthew Longo
    Pages 199-206

About this book

Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Staphylococcus Epidermidis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Reviews

From the reviews:

This 19-chapter book is entirely focused on everything you might ever want to know about Staphylococcus epidermidis. … This book would be of great interest to anyone working with Staphylococcus epidermidis, particularly those in a research setting. … many chapters come fully equipped with detailed experimental protocols ready for you to try in your laboratory. If you have any interest in any aspect of Staphylococcus epidermidis, you will want to read and likely own this book.” (Valerie L. Ng, Doody’s Book Reviews, February, 2014)

Editors and Affiliations

  • Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, USA

    Paul D. Fey

Bibliographic Information

  • Book Title: Staphylococcus Epidermidis

  • Book Subtitle: Methods and Protocols

  • Editors: Paul D. Fey

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-736-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-735-8Published: 13 November 2013

  • Softcover ISBN: 978-1-4939-6053-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-736-5Published: 13 November 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 208

  • Number of Illustrations: 12 b/w illustrations, 18 illustrations in colour

  • Topics: Infectious Diseases, Bacteriology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access