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  • © 2012

Neisseria meningitidis

Advanced Methods and Protocols

  • Provides advanced, cutting-edge techniques for the study of the meningococcus
  • Builds upon the foundation of previous books in the series to supply the most current methods and protocols
  • Includes expert tips and implementation advice to ensure successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 799)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
  2. Neisseria meningitidis: Biology, Microbiology, and Epidemiology

    • Nadine G. Rouphael, David S. Stephens
    Pages 1-20
  3. Classification and Pathogenesis of Meningococcal Infections

    • Petter Brandtzaeg, Marcel van Deuren
    Pages 21-35
  4. Expression, Purification, and Crystallization of Neisserial Outer Membrane Proteins

    • Muhammad Saleem, Jeremy Moore, Jeremy P. Derrick
    Pages 91-106
  5. Genome-Scale Metabolic Models: Reconstruction and Analysis

    • Gino J. E. Baart, Dirk E. Martens
    Pages 107-126
  6. TMT Labelling for the Quantitative Analysis of Adaptive Responses in the Meningococcal Proteome

    • Karsten Kuhn, Christian Baumann, Jan Tommassen, Thorsten Prinz
    Pages 127-141
  7. Meningococcal Ligands and Molecular Targets of the Host

    • Darryl J. Hill, Mumtaz Virji
    Pages 143-152
  8. In Vivo Imaging of Meningococcal Disease Dynamics

    • Hong Sjölinder, Ann-Beth Jonsson
    Pages 153-168
  9. Methods for Studying Neisseria meningitidisBiofilms

    • Michael A. Apicella, Jianqiang Shao, R. Brock Neil
    Pages 169-184
  10. Techniques to Measure Pilus Retraction Forces

    • Nicolas Biais, Dustin Higashi, Magdalene So, Benoit Ladoux
    Pages 197-216
  11. Human Dendritic Cell Culture and Bacterial Infection

    • Hannah E. Jones, Nigel Klein, Garth L. J. Dixon
    Pages 217-235
  12. Hydrogen–Deuterium Exchange Coupled to Mass Spectrometry to Investigate Ligand–Receptor Interactions

    • Jessmi M. L. Ling, Leslie Silva, David C. Schriemer, Anthony B. Schryvers
    Pages 237-252
  13. Visualising PAMP–PRR Interactions Using Nanoscale Imaging

    • Kathy Triantafilou, Martha Triantafilou
    Pages 253-266
  14. Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells

    • Biju Joseph, Matthias Frosch, Christoph Schoen, Alexandra Schubert-Unkmeir
    Pages 267-293

About this book

Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors.  Neisseria meningitidis: Advanced Methods and Protocols offers a collection of methods and protocols that reflect the development and refinement of several new technologies applied to the meningococcus as presented by expert researchers. Several of the chapters in this book describe methods that rely on the collection of complete sets of biological data, for example, using the genome to generate transcriptomes, proteomes, and metabolomes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Neisseria meningitidis: Advanced Methods and Protocols can be readily used to study other pathogens and diseases and should have broad appeal to clinical and non-clinical scientists alike; however, there is still much to learn about the meningococcus and its complex nature, which in turn continues to reveal crucial details about human biology as well.

Editors and Affiliations

  • Medical School, Molecular Microbiology and Infection, University of Southampton, Southampton, United Kingdom

    Myron Christodoulides

Bibliographic Information

  • Book Title: Neisseria meningitidis

  • Book Subtitle: Advanced Methods and Protocols

  • Editors: Myron Christodoulides

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-346-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-345-5Published: 13 October 2011

  • Softcover ISBN: 978-1-4939-5860-3Published: 23 August 2016

  • eBook ISBN: 978-1-61779-346-2Published: 14 October 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 427

  • Topics: Infectious Diseases, Bacteriology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access