Hepatitis C

1. Front Matter

   Pages i-xxi

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2. Detection and Quantification of HCV

   1. Front Matter

      Pages 1-1

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   2. Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays

      • Helene Le Guillou-Guillemette, Francoise Lunel-Fabiani

      Pages 3-14

   3. Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen

      • Filippo Ansaldi, Giancarlo Icardi

      Pages 15-23

   4. Immunohistochemical Detection of HCV Proteins in Liver Tissue

      • Oivier Galy, Isabelle Vincent, Philippe Chevallier, Nicole Lyandrat, Isabelle Chemin

      Pages 25-30

3. HCV Nomenclature, Genotyping, and Quasispecies

   1. Front Matter

      Pages 31-31

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   2. Nomenclature and Numbering of the Hepatitis C Virus

      • Carla Kuiken, Peter Simmonds

      Pages 33-53

   3. A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates

      • Kathryn J. Rolfe, Tim G. Wreghitt, Graeme J. M. Alexander, Martin D. Curran

      Pages 55-71

   4. Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry

      • Carmen Yea, Melissa Ayers, Raymond Tellier

      Pages 73-81

4. HCV Replication in Vitro

   1. Front Matter

      Pages 83-83

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   2. Purification and Crystallization of NS5A Domain I of Hepatitis C Virus

      • Joseph Marcotrigiano, Timothy Tellinghuisen

      Pages 85-94

   3. NS5A Phosphorylation and Hyperphosphorylation

      • Petra Neddermann

      Pages 95-110

   4. Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies

      • Stefania Di Marco, Andrea Carfí

      Pages 111-124

   5. Structural and Functional Analysis of the HCV p7 Protein

      • Nathalie Saint, Roland Montserret, Christophe Chipot, François Penin

      Pages 125-143

   6. HCV Replicons: Overview and Basic Protocols

      • Volker Lohmann

      Pages 145-163

   7. Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA

      • Timothy L. Tellinghuisen, Brett D. Lindenbach

      Pages 165-175

   8. Studying HCV RNA Synthesis In Vitro with Replication Complexes

      • Wengang Yang, Mingjun Huang

      Pages 177-184

   9. Proteomics Study of the Hepatitis C Virus Replication Complex

      • Kyungsoo Chang, Tianyi Wang, Guangxiang Luo

      Pages 185-193

   10. Investigation of the Hepatitis C Virus Replication Complex

       • Volker Brass, Rainer Gosert, Darius Moradpour

       Pages 195-209

   11. Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease

       • Kui Li

       Pages 211-226

When I was ?rst approached by Dr. Hengli Tang about writing the foreword to this book, the second edition of Hepatitis C Virus, I was a little puzzled: Book forewords are penned by persons far more famous than I am. Surely there were better people to write this, with a handful of the true visionaries in the ?eld immediately coming to my mind. However, after some thought and self-re?ection, I realized that I might just be the right person for the job. I remember the publication of the ?rst edition of Hepatitis C Virus in 1998, the year I began reading about hepatitis C virus with great interest. I purchased a copy of the ?rst edition from my meager graduate student stipend and read it from cover to cover, along with a variety of hepatitis C publications, to try to get a feel if this hepatitis C virus ?eld was something I might want to work on in the future. At the time, the ?rst edition was among the most comprehensive collection of hepatitis C protocols and methods available in one volume. I was amazed at the progress that had been made in hepatitis C research, and even more amazed as I learned more about the experimental challenges facing the ?eld at that time.

• Antigen
• Genotypes
• HCV research
• Hepatitis
• Quasispecies
• RNA replication
• Virus
• infection
• infectious
• hepatology
• infectious diseases

From the reviews of the second edition: “This second edition contains much that was not available for the first edition; replicons, tissue culture infection systems, infectious pseudotype particles, etc. … The chapters are authored by acknowledged experts and are authoritatively written. … Of particular value are the notes sections which highlight practical pitfalls and how to avoid them. Given the size of the hepatitis C community … this book will be a success and should adorn the shelves of many laboratories.” (David J. Rowlands, Microbiology Today, July, 2009)

• Florida State University, Tallahassee, USA

  Hengli Tang

Policies and ethics