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Medicine - Internal Medicine | Animal Influenza Virus

Animal Influenza Virus

Series: Methods in Molecular Biology, Vol. 1161

Spackman, Erica (Ed.)

2nd ed. 2014, XII, 425 p. 58 illus., 48 illus. in color.

A product of Humana Press
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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Avian Influenza Virus, Second Edition aims to provide the essential methods used in working with animal influenza viruses, and to compile more advanced information that will guide the user in designing influenza studies. Influenza A viruses are among the most important pathogens for humans, food animals and companion animals. Research and diagnostics with animal influenza viruses are critical to animal health and it should be recognized that the needs and goals of animal agriculture and veterinary medicine are not always the same as those of public health.  This volume sorts out the differences in the structure of the poultry, swine and equine industries, the biological differences of influenza virus from each animal group, and provides host, strain and lineage specific guidance and procedures. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Avian Influenza Virus, Second Edition seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of these important pathogens.

Content Level » Professional/practitioner

Keywords » animal influenza - avian influenza - equine influenza - hemagglutination assay - influenza A virus

Related subjects » Biomedical Sciences - Internal Medicine

Table of contents 

Part I. General


1. Hemagglutination Assay for Influenza Virus

                Mary Lea Killian


2. Hemagglutination-Inhibition Assay for Influenza Virus Subtype Identification and the Detection and Quantitation of Serum Antibodies to Influenza Virus

                Janice. C. Pedersen


3. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Virus Subtype or Neuraminidase Antibody Specificity

                Janice. C. Pedersen


4. Reverse Genetics of  Influenza Virus

                Chang-Won Lee


5. Immunohistochemical Staining of Influenza Virus in Tissues

                Mary J. Pantin-Jackwood


Part II. Avian Influenza


6. A Brief Introduction to Avian Influenza Virus

                Erica Spackman


7. Wild Bird Surveillance for Avian Influenza Virus

                Justin D. Brown, Rebecca Poulson, and David E. Stallknecht


8. Avian Influenza Virus Sample Types, Collection and Handling

                Mary Lea Killian


9. Avian Influenza Virus RNA Extraction

                Erica Spackman and Scott A. Lee


10. Avian Influenza Virus Detection and Quantitation by Real-Time RT-PCR

                Erica Spackman


11. Influenza Subtype Identification with Molecular Methods

                Erica Spackman


12. Avian Influenza Virus Isolation, Propagation and Titration in Embryonated Chicken Eggs

                Erica Spackman and Mary Lea Killian


13. Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus

                Terra A. Jenson


14. Detection of Influenza A Antibodies in Avian Serum Samples by ELISA

                Len Chappell, Mary Lea Killian, and Erica Spackman


15. Conducting Influenza Virus Pathogenesis Studies in Avian Species

                Carol Cardona, David A. Halvorson, Justin Brown, Jeffrey Hall, and Mary Pantin-Jackwood


16. Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultry

                David E. Swayne


17. Detection of Cell Mediated Immune Response to Avian Influenza Viruses

                Darrell R. Kapczynski


18. Characterization of Cytokine Expression Induced by Avian Influenza Virus Infection with Real-Time RT-PCR

                Darrell R. Kapczynski, Hai Jun Jiang, and Michael H. Kogut


19. Newcastle Disease Virus Detection and Differentiation from Avian Influenza

                Patti J. Miller and Mia Kim Torchetti


20. A Brief Introduction to Influenza A Virus in Swine

                Amy L. Vincent, Kelly M. Lager, and Tavis K. Anderson


21. Sample Types, Collection, and Transport for Influenza A Viruses of Swine

                Marie Culhane and Susan Detmer


22. Isolation of Swine Influenza Virus in Cell Cultures and Embryonated Chicken Eggs

                Jianqiang Zhang and Phillip C. Gauger


23. RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-Time RT-PCR

                Jianqiang Zhang and Karen M Harmon


24. Hemagglutinin Inhibition Assay with Swine Sera

                Pravina Kitikoon, Phillip C. Gauger, and Amy L. Vincent


25. Enzyme-Linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype Specific IgG and IgA Whole Virus Antibody to Influenza A Virus in Swine

                Phillip C. Gauger, Crystal L. Loving, and Amy L. Vincent


26. Serum Virus Neutralization Assay for Detection and Quantitation of Serum Neutralizing Antibodies to Influenza A Virus in Swine

                Phillip C. Gauger and Amy L. Vincent


27. Microneutralization Assay for Swine Influenza Virus in Swine Serum

                Pravina Kitikoon and Amy L. Vincent


28. Analyzing Swine Sera for Functional Antibody Titers Against Influenza A Neuraminidase Proteins Using an Enzyme-Linked Lectin Assay (ELLA)

                Matthew R. Sandbulte and Maryna C. Eichelberger


29. Antibody Secreting Cell Assay for Influenza A Virus in Swine

                Pravina Kitikoon, Crystal L. Loving, and Amy L. Vincent


30. In vivo Models for Pathotyping and Vaccine Efficacy for Swine Influenza

                Kelly M. Lager and Amy L. Vincent


Part III. Equine Influenza


31. A Brief Introduction to Equine Influenza and Equine Influenza Viruses

                Thomas M. Chambers


32. Equine Influenza Diagnosis: Sample Collection and Transport

                Thomas M. Chambers and Stephanie E. Reedy


33. RNA Extraction from Equine Samples for Equine Influenza Virus

                Udeni B. R. Balasuriya


34. Type A Influenza Virus Detection from Horses by Real-Time RT-PCR and Insulated Isothermal RT-PCR

                Udeni B. R. Balasuriya


35. Equine Influenza Culture Methods

                Thomas M. Chambers and Stephanie E. Reedy


36. Equine Influenza Serological Methods

                Thomas M. Chambers and Stephanie E. Reedy

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