Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1138)
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Table of contents (25 protocols)
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Front Matter
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Modern Quantitative Virological Methods
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Front Matter
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Reverse Genetic Systems to Study Virus Replication and Evolution
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Front Matter
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Modern Methods to Study Conformation of RNA Proteins and Their Molecular Interactions
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Front Matter
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About this book
Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.
Editors and Affiliations
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Georgetown University Medical Center Dept. of Microbiology and Immunology, Washington, USA
Radhakrishnan Padmanabhan
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Duke-NUS GMS Singapore Emerging Infectious Diseases, Singapore, Singapore
Subhash G. Vasudevan
Bibliographic Information
Book Title: Dengue
Book Subtitle: Methods and Protocols
Editors: Radhakrishnan Padmanabhan, Subhash G. Vasudevan
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-0348-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-4939-0347-4Published: 03 April 2014
Softcover ISBN: 978-1-4939-4371-5Published: 23 August 2016
eBook ISBN: 978-1-4939-0348-1Published: 02 April 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 403
Number of Illustrations: 33 b/w illustrations, 49 illustrations in colour
Topics: Infectious Diseases, Virology, Laboratory Medicine