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Life Sciences - Systems Biology and Bioinformatics | Synthetic Gene Networks - Methods and Protocols

Synthetic Gene Networks

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 813

Weber, Wilfried, Fussenegger, Martin (Eds.)

2012, XI, 393p. 67 illus., 3 illus. in color.

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

The rapid expansion of synthetic biology is due to the design and construction of synthetic gene networks that have opened many new avenues in fundamental and applied research. Synthetic Gene Networks: Methods and Protocols provides the necessary information to design and construct synthetic gene networks in different host backgrounds. Divided into four convenient sections, this volume focuses on design concepts to devise synthetic gene networks and how mathematical models can be applied to the predictable engineering of desired network features. The volume continues by highlighting the construction and validation of biologic tools, describing strategies to optimize and streamline the host cell for optimized network performance, and covering how optimally designed gene networks can be implemented in a large variety of host cells ranging from bacteria over yeast and insect cells to plant and mammalian cell culture. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Synthetic Gene Networks: Methods and Protocols serves as an invaluable resource for established biologists, engineers, and computer scientists or novices just entering into the rapidly growing field of synthetic biology

Content Level » Professional/practitioner

Keywords » RNA-based networks - complex cellular phenotypes - complex lentiviral gene regulation - isogenic human cell lines - synthetic biology - synthetic gene networks - transcriptional logic

Related subjects » Systems Biology and Bioinformatics

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