DNA-based markers in plants

1. Front Matter

   Pages i-x

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2. Some concepts and new methods for molecular mapping in plants

   • Benjamin Burr

   Pages 1-7

3. RFLP technology

   • Gary Kochert

   Pages 8-38

4. Constructing a plant genetic linkage map with DNA markers

   • Nevin Dale Young

   Pages 39-57

5. Mapping quantitative trait loci

   • Steven J. Knapp

   Pages 58-96

6. Breeding multigenic traits

   • Charles W. Stuber

   Pages 97-115

7. Nuclear DNA markers in systematics and evolution

   • Richard Whitkus, John Doebley, Jonathan F. Wendel

   Pages 116-141

8. Introduction: molecular marker maps of major crop species

   • Ronald L. Phillips, Indra K. Vasil

   Pages 142-143

9. Molecular maps of alfalfa

   • E. C. Brummer, C. S. Echt, T. J. McCoy, K. K. Kidwell, T. C. Osborn, G. B. Kiss et al.

   Pages 144-158

10. An integrated RFLP map of Arabidopsis thaliana

    • Howard M. Goodman, Susan Hanley, Sam Cartinhour, J. Michael Cherry, Brian Hauge, Elliot Meyerowitz et al.

    Pages 159-162

11. RFLP maps of barley

    • Andris Kleinhofs, Andrzej Kilian

    Pages 163-198

12. DNA-based marker maps of Brassica

    • Carlos F. Quiros, Jinguo Hu, Maria Jose Truco

    Pages 199-222

13. Genetic mapping in lettuce

    • Richard W. Michelmore, Richard V. Kesseli, Edward J. Ryder

    Pages 223-239

14. RFLP maps of maize

    • Edward H. Coe, Jack M. Gardiner

    Pages 240-245

15. RFLP map of peanut

    • Tracy Halward, H. Thomas Stalker, Gary Kochert

    Pages 246-260

16. Phaseolus vulgaris: the common bean

    • C. Eduardo Vallejos

    Pages 261-270

17. RFLP map of the potato

    • Christiane Gebhardt, Enrique Ritter, Francesco Salamini

    Pages 271-285

18. Rice molecular map

    • S. D. Tanksley

    Pages 286-290

19. Generation of a genetic map for Sorghum bicolor

    • Jeffrey L. Bennetzen, Admasu Melake-Berhan

    Pages 291-298

20. RFLP map of soybean

    • Randy C. Shoemaker

    Pages 299-309

The double helix architecture of DNA was elucidated in 1953. Twenty years later, in 1973, the discovery of restriction enzymes helped to create recombinant DNA molecules in vitro. The implications of these powerful and novel methods of molecular biology, and their potential in the genetic manipulation and improvement of microbes, plants and animals, became increasingly evident, and led to the birth of modern biotechnology. The first transgenic plants in which a bacterial gene had been stably integrated were produced in 1983, and by 1993 transgenic plants had been produced in all major crop species, including the cereals and the legumes. These remarkable achievements have resulted in the production of crops that are resistant to potent but environmentally safe herbicides, or to viral pathogens and insect pests. In other instances genes have been introduced that delay fruit ripening, or increase starch content, or cause male sterility. Most of these manipulations are based on the introduction of a single gene - generally of bacterial origin - that regulates an important monogenic trait, into the crop of choice. Many of the engineered crops are now under field trials and are expected to be commercially produced within the next few years. The early successes in plant biotechnology led to the realization that further molecular improvement of plants will require a thorough understanding of the molecular basis of plant development, and the identification and character­ ization of genes that regulate agronomically important multi genic traits.

• DNA
• Liana
• arabidopsis thaliana
• development
• evolution
• gene
• genome
• molecular techniques
• plant
• plants
• wheat

• Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, USA

  Ronald L. Phillips

• Laboratory of Plant Cell and Molecular Biology, University of Florida, Gainesville, USA

  Indra K. Vasil

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