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Deals with molecular tools in plant breeding, molecular marker assisted breeding, gene silencing, functional genomics and genetic engineering
The first edition of this book, "Molecular techniques in crop improvement" published in 2002 provided comprehensive information on the latest tools and techniques of molecular genetics and applications in crop improvement, and highlighted molecular genetics from the perspective of plant breeders. Since then, major advances have been made in molecular tagging of genes/QTLs governing complex agronomic traits, identification of candidate genes and in applying marker assisted breeding for tolerance to biotic and abiotic stresses and quality traits. Recent advances in transgenic technologies, genome sequencing and functional genomics offer tremendous opportunities to support plant breeding programs. We have covered new developments in molecular biology and their potential applications in plant breeding in this second edition. The book has a total of 31 chapters and divided into 4 sections: A) Plant breeding in the genomics era, B) Molecular markers and their application, C) Genomics, and D) Transgenic technologies. The book features major topics, which are QTL analysis, comparative genomics, functional genomics, bioinformatics, DNA marker technology automation, gene-based marker systems, , application of molecular markers for tolerance to biotic and abiotic stresses as well as in germplasm conservation, gene pyramiding, gene silencing, TILLING, CISGENESIS, microarray, metabolomics, proteomics, transcriptomics, microRNAs, marker-free transformation, gene targeting/homologous recombination, and genetic engineering. This book will be especially useful to scientists engaged in molecular genetics and plant breeding. It will also be a valuable book for the graduate and post graduate students specialising in crop science, genetics, plant breeding and biotechnology.
A. Plant Breeding in the genomics era:1.QTL analysis for plant breeding; Maria J. Asins, Guillermo P. Bernet, Irene Villalta and Emilio A. Carbonelli.- 2.Comparative genomics in crop plants; Mehboob-ur-Rahman and Andrew H. Paterson.- 3.Functional genomics for crop improvement; Seedhabadee Ganeshan, Pallavi Sharma and Ravindra N. Chibbar.- 4.Bioinformatics tools for crop research and breeding; Jayashree B and Dave Hoisington.-
B. Molecular markers and their application:5.Gene-based marker systems in plants: high throughput approaches for gene discovery and genotyping; Rajeev K Varshney.- 6.Automation of DNA marker analysis for molecular breeding in crops; Christophe Dayteg, Stine Tuvesson.- 7.Pyramiding transgenes for enhancing tolerance to abiotic and biotic stresses; Raveendran Muthurajan and Ponnuswami Balasubramanian.- 8.Molecular markers for breeding disease resistance; Ana M. Torres.- 9.Molecular markers based approaches for drought tolerance; Deepmala Sehgal and Rattan Yada.- 10. Molecular markers for characterizing and conserving crop plant germplasm; G. Barcaccia.-
C. Genomics:11.Rice Genomics -Gateway To Future Cereal Improvement; Narayana M. Upadhyaya And Elizabeth S. Dennis.- 12.Genomics for wheat improvement; Michael G. Francki.- 13.TILLING for mutations in model plants and crops; Zerihun Tadele, Chikelu Mba and Bradley J. Till.- 14.Microarray analysis for studying abiotic stress responses in plants; Motoaki Seki, Masanori Okamoto, Akihiro Matsui, Jong-Myong Kim, Yukio Kurihara, Junko Ishida, Taeko Morosawa, Makiko Kawashima, Taiko Kim To and Kazuo Shinozaki.- 15.Roles of microRNAs in plant abiotic stress; Ricky Lewis, Venugopal Mendu, David McNear and Guiliang Tang.- 16.Molecular Tools For Enhancing Salinity Tolerance In Plants; Jesus Cuartero, Maria C. Bolarin, Vicente Moreno And Benito Pineda.- 17.DNA Microarray As Part Of A Genomic-Assisted Breeding Approach-Gateway To Improve Nutritional Quality Of Barley; Eva Vincze And SteveBowra.- 18.Unravelling Gene Function Through Mutagenesis; Andrea Hricová, Pedro Robles And Víctor Quesada.- 19.Techniques Of Plant Proteomics; Ludovít Skultéty, Maxym Danchenko, Anna Pretová And Martin Hajduch.- 20.Metabolomics: Novel Tool For Studying Complex Biological Systems; Federica Maltese And Robert Verpoorte.- 21.Transcriptomic analysis of multiple environmental stresses in plants; Niranjani Jambunathan, Michael Puckette and Ramamurthy Mahalingam.-
D. Transgenic Technologies:22.Marker-free targeted transformation; Hiroyasu Ebinuma and Kazuya Nanto.- 23.Promoter trapping in plants using T-DNA mutagenesis; T. Srinivasan and Dipnarayan Sah.- 24.Plant genome engineering using zinc finger nucleases; Sandeep Kumar and William F. Thompson.- 25.CISGENESIS- Next step in classical plant breeding; Evert Jacobsen and Henk J. Schouten.- 26.Gene stacking; E. Douglas and C. Halpin.- 27.Gene Silencing; Sunee Kertbundit, Miloslav Jurícek and Timothy C. Hall.- 28.Plant RNAi and crop improvement; Masayuki Isshiki and Hiroaki Kodama.- 29.Metabolomics in fruit development; Kati Hanhineva and Asaph Aharoni.- 30.Genetic engineering in floriculture; Yoshikazu Tanaka and Ryutaro Aida.- 31.Transgenesis and genomics in forage crops; Toshihiko Yamada, Ken-ichi Tamura, Xun Wang, and Yukiko Aoyagi.-