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Plant Cell, Tissue and Organ Culture

Fundamental Methods

  • Book
  • © 1995

Overview

Editors:
  1. Oluf L. Gamborg

    1. University of California, Davis, Belmont, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Gregory C. Phillips

    1. Molecular Biology Program, Department of Agronomy and Horticulture, New Mexico State University, Las Cruces, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (22 chapters)

  1. Front Matter

    Pages I-XXIV
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  2. Facilities and Technology Requirements for Laboratory Operations

    1. Front Matter

      Pages 1-1
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    2. Laboratory Facilities, Operation, and Management

      • Oluf L. Gamborg, Gregory C. Phillips
      Pages 3-20

    3. Media Preparation and Handling

      • Oluf L. Gamborg, Gregory C. Phillips
      Pages 21-34

    4. Sterile Techniques

      • Oluf L. Gamborg, Gregory C. Phillips
      Pages 35-42

  3. Basic Plant Regeneration and Plant Propagation Procedures

    1. Front Matter

      Pages 43-43
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    2. Micropropagation by Proliferation of Axillary Buds

      • Gregory C. Phillips, John F. Hubstenberger
      Pages 45-54

    3. Adventitious Shoot Proliferation

      • Gregory C. Phillips, John F. Hubstenberger, Elizabeth E. Hansen
      Pages 55-65

    4. Plant Regeneration by Organogenesis from Callus and Cell Suspension Cultures

      • Gregory C. Phillips, John F. Hubstenberger, Elizabeth E. Hansen
      Pages 67-79

    5. Plant Regeneration from Callus and Cell Suspension Cultures by Somatic Embryogenesis

      • Gregory C. Phillips, John F. Hubstenberger, Elizabeth E. Hansen
      Pages 81-90

    6. Direct Somatic Embryogenesis

      • John J. Finer
      Pages 91-102

    7. Conifer Somatic Embryogenesis, Embryo Development, Maturation Drying, and Plant Formation

      • Stephen M. Attree, Larry C. Fowke
      Pages 103-113

  4. Applications of Organ Culture Systems

    1. Front Matter

      Pages 115-115
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    2. Meristem Culture for Virus Elimination

      • Steven A. Slack, Laura A. Tufford
      Pages 117-128

    3. Embryo Culture and Embryo Rescue for Wide Cross Hybrids

      • Ching-Yeh Hu, Maria Helena B. Zanettini
      Pages 129-141

    4. Anther Culture for Doubled Haploid Production

      • Timothy P. Croughan
      Pages 143-154

    5. Microspore Culture for Haploid Plant Production

      • A. M. R. Ferrie, W. A. Keller
      Pages 155-164

  5. Protoplasts and Transformation Procedures

    1. Front Matter

      Pages 165-165
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    2. Protoplast Isolation and Culture

      • Stephen C. Gleddie
      Pages 167-180

    3. Agrobacterium-Mediated Transformation

      • Ebrahim Firoozabady, Adelheid R. Kuehnle
      Pages 181-195
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Keywords

About this book

This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books.
It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Reviews

"...provides assistance in acquiring those skills that are critical for plant tissue culture and plant biotechnology research." Horticultural Science "...a well produced book with comprehensive details on the major techniques used in plant biotechnology currently. The book is well organised and well illustrated." Australasian Biotechnology

Editors and Affiliations

  • University of California, Davis, Belmont, USA

    Oluf L. Gamborg

  • Molecular Biology Program, Department of Agronomy and Horticulture, New Mexico State University, Las Cruces, USA

    Gregory C. Phillips

Bibliographic Information

  • Book Title: Plant Cell, Tissue and Organ Culture

  • Book Subtitle: Fundamental Methods

  • Editors: Oluf L. Gamborg, Gregory C. Phillips

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-79048-5

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1995

  • Softcover ISBN: 978-3-642-48974-7Published: 03 October 2013

  • eBook ISBN: 978-3-642-79048-5Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XXIV, 359

  • Number of Illustrations: 131 b/w illustrations, 10 illustrations in colour

  • Topics: Plant Sciences, Agriculture, Forestry, Biochemistry, general, Biotechnology

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