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Legume Genomics

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Ray J. Rose

    1. CILR, The University of Newcastle Environmental & Life Sciences, Callaghan, Australia

    View editor publications

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  • Features cutting-edge methods and protocols examining genes and gene interactions in legumes
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1069)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Model Legume Genomes

    • Steven B. Cannon
    Pages 1-14

  3. Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization

    • René Geurts, Hans de Jong
    Pages 15-24

  4. Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes

    • Shaun J. Curtin, Justin E. Anderson, Colby G. Starker, Nicholas J. Baltes, Dhananjay Mani, Daniel F. Voytas et al.
    Pages 25-42

  5. RNA-Seq for Transcriptome Analysis in Non-model Plants

    • Rohini Garg, Mukesh Jain
    Pages 43-58

  6. Functional Analysis of Legume Genome Arrays

    • Nicolas Goffard, Georg Weiller
    Pages 59-66

  7. Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing

    • Tian-Zuo Wang, Wen-Hao Zhang
    Pages 67-80

  8. Determining Abundance of MicroRNAs and Other Small RNAs in Legumes

    • Cecilia Contreras-Cubas, Alejandra A. Covarrubias, José Luis Reyes
    Pages 81-92

  9. Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines

    • Juan Carlos Serrani Yarce, Hee-Kyung Lee, Million Tadege, Pascal Ratet, Kirankumar S. Mysore
    Pages 93-100

  10. Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection

    • Maria Carelli, Ornella Calderini, Francesco Panara, Andrea Porceddu, Ilaria Losini, Pietro Piffanelli et al.
    Pages 101-118

  11. High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants

    • Dorian Fabian UrbaÅ„ski, Anna MaÅ‚olepszy, Jens Stougaard, Stig Uggerhøj Andersen
    Pages 119-146

  12. Isolation and Forward Genetic Analysis of Developmental Genes in Pea

    • James L. Weller, Valérie F. G. Hecht, Frances C. Sussmilch
    Pages 147-161

  13. Gene Silencing in Medicago truncatula Roots Using RNAi

    • Daniela S. Floss, Alexa M. Schmitz, Colby G. Starker, J. Stephen Gantt, Maria J. Harrison
    Pages 163-177

  14. Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius)

    • Islam Shahidul, Huaan Yang, Guijun Yan
    Pages 179-201

  15. Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens

    • Youhong Song, Kim E. Nolan, Ray J. Rose
    Pages 203-214

  16. Transient Gene Expression in Medicago truncatula Leaves via Agroinfiltration

    • Kelsey Picard, Robyn Lee, Roger Hellens, Richard Macknight
    Pages 215-226

  17. Subcellular Localization of Transiently Expressed Fluorescent Fusion Proteins

    • David A. Collings
    Pages 227-258

  18. Proteomics and the Analysis of Nodulation

    • Nijat Imin
    Pages 259-269

  19. Phosphoproteomic Analysis of Peptides

    • Mark A. Baker
    Pages 271-277

  20. Plant Metabolomics: From Experimental Design to Knowledge Extraction

    • Amit Rai, Shivshankar Umashankar, Sanjay Swarup
    Pages 279-312
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Keywords

About this book

Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.

Editors and Affiliations

  • CILR, The University of Newcastle Environmental & Life Sciences, Callaghan, Australia

    Ray J. Rose

Bibliographic Information

  • Book Title: Legume Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Ray J. Rose

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-613-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-612-2Published: 01 September 2013

  • Softcover ISBN: 978-1-4939-6024-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-613-9Published: 31 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 316

  • Number of Illustrations: 30 b/w illustrations, 23 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics, Gene Function

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