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Life Sciences - Plant Sciences | G Protein-Coupled Receptor Signaling in Plants - Methods and Protocols

G Protein-Coupled Receptor Signaling in Plants

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1043

Running, Mark P. (Ed.)

2013, X, 162 p. 19 illus., 14 illus. in color.

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  • Explores techniques to examine the roles of GCRPs and small GTPases in key plant biological processes
  • Features step-by-step protocols that are ready for the lab
  • Includes expert tips and key implementation advice to ensure quality results

Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins.  Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.

Content Level » Professional/practitioner

Keywords » Cell signaling - G protein-coupled receptors - GPCRs - Heterotrimeric G proteins - Lipid modification - Plant signal transduction - Rab-GTPases - Rop GTPases - Small GTPases

Related subjects » Biochemistry & Biophysics - Plant Sciences

Table of contents 

1. Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs

            Timothy E. Gookin and Jannick D. Bendtsen


2. Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins

            Swarup Roy Choudhury, Corey S. Westfall, Dieter Hackenberg, and Sona Pandey


3. Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana

            Jian Zhao and Xuemin Wang


4. Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants

            Zhaoqing Jin, Wellington Muchero, and Jin-Gui Chen


5. Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis

            Kiwamu Tanaka, Jeongmin Choi, and Gary Stacey


6. Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis

            Guojing Li, Xiujuan Zhang, Dongli Wan, Shuqun Zhang, and Yiji Xia


7. Fusarium oxysporum Infection Assays in Arabidopsis

            Yuri Trusov, David Chakravorty, and Jose Ramon Botella


8. Analysis of Unfolded Protein Response in Arabidopsis

            Yani Chen and Federica Brandizzi


9. Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis

            Wenjuan Wu and Jirong Huang


10. G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings

            Danielle A. Orozco-Nunnelly, Lon S. Kaufman, and Katherine M. Warpeha


11. Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana

            Xingyun Qi and Huanquan Zheng


12. In Vivo Localization in Arabidopsis Protoplasts and Root Tissue

            Myoung Hui Lee, Yongjik Lee, and Inhwan Hwang


13. Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography–Coupled Mass Spectrometry

            Nadav Sorek, Amir Akerman, and Shaul Yalovsky


14. In Vitro Myristoylation Assay of Arabidopsis Proteins

            Xuehui Feng, Wan Shi, Xuejun Wang, and Mark P. Running


15. Assaying Protein S-Acylation in Plants

            Piers A. Hemsley


16. In Vitro Prenylation Assay of Arabidopsis Proteins

            Wan Shi, Qin Zeng, and Mark P. Running

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