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Life Sciences - Plant Sciences | Cyclic Nucleotide Signaling in Plants - Methods and Protocols

Cyclic Nucleotide Signaling in Plants

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1016

Gehring, Chris (Ed.)

2013, X, 273 p. 41 illus., 25 illus. in color.

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  • Includes cutting-edge methods and protocols in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.



Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling.

Content Level » Professional/practitioner

Keywords » bioinformatic methods - cAMP - cGMP - cyclic nucleotide gated channels - cyclic nucleotide phosphodiesterases - cyclic nucleotide research - cyclic nucleotide transients - nitric oxide in plant tissues - nucleotide cyclases - oxygen species - photosynthetic responses - plant signal transduction

Related subjects » Biochemistry & Biophysics - Plant Sciences

Table of contents 

1: Molecular Methods for the Study of Signal Transduction in Plants

Helen R. Irving and Chris Gehring


2: Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains

Oziniel Ruzvidzo, Bridget T. Dikobe, David T. Kawadza, Grace H. Mabadahanye, Patience Chatukuta, Lusisizwe Kwezi


3: Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS

Heike Burhenne and Volkhard Kaever


4: Determination of ADP-ribosyl Cyclase Activity, Cyclic ADP-ribose and Nicotinamide Adenine Dinucleotide Phosphate in Tissue Extracts

Richard M. Graeff and Hon Cheung Lee


5: In vivo Imaging of cGMP in Plants

Jean-Charles Isner and Frans J.M. Maathuis


 6: Characterization of Heterologously Expressed Transporter Genes by Patch- and Voltage-Clamp Methods: Application to Cyclic Nucleotide-Dependent Responses

Fouad Lemtiri-Chlieh and Rashid Ali


7: Non-invasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides

Natalia Maria Ordoñez, Lana Shabala, Chris Gehring, and Sergey Shabala


 8: Calcium Imaging of the Cyclic Nucleotide Response

Martin R. McAinsh, Stephen K. Roberts and Lyudmila V. Dubovskaya



9: Identification and Quantification of Signal Molecule-dependent Protein Phosphorylation

Arnoud Groen, Ludivine Thomas, Kathryn Lilley and Claudius Marondedze



10: Comparative Gel-based (Phospho)Proteomics in Response to Signaling Molecules

Claudius Marondedze, Kathryn Lilley and Ludivine Thomas

11: An Affinity Pull-down Approach to Identify the Plant Cyclic Nucleotide Interactome

Lara Donaldson and Stuart Meier


12: Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activity

Victor Muleya, Janet I. Wheeler and Helen R. Irving


13: Computational Identification of Candidate Nucleotide Cyclases in Higher Plants

Aloysius Wong and Chris Gehring


14: Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions

Alice K. Zelman, Adam Dawe and Gerald A. Berkowitz


15: Inferring biological functions of guanylyl cyclases with computational methods

May Alqurashi and Stuart Meier


16: Identification and Characterization of Cyclic Nucleotide Phosphodiesterases

Erin B. Purcell and Rita Tamayo


17: Detection of reactive oxygen species down-stream of cyclic nucleotide signals in plants

Robin K. Walker and Gerald A. Berkowitz


18: Measurement of Nitric Oxide in Plant Tissue using Difluorofluorescein and Oxyhaemoglobin

Ndiko Ludidi


 19: Infra-red Gas Analysis (IRGA) Technique for the Study of the Regulation of Photosynthetic Responses

Alex Valentine, Oziniel Ruzvidzo, Aleysia Kleinert, Yun Kang and Vagner Bennedito



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