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Life Sciences - Plant Sciences | Plant Cold Acclimation - Methods and Protocols

Plant Cold Acclimation

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1166

Hincha, Dirk K., Zuther, Ellen (Eds.)

2014, XI, 282 p. 75 illus., 49 illus. in color.

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  • Includes cuttign-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts.

Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties.  Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches.

Content Level » Professional/practitioner

Keywords » breeding - cold - cold acclimation - freezing tolerance - genetics - low temperature tolerance - non-freezing temperatures - physiology or molecular biology - plants - supercooling

Related subjects » Agriculture - Plant Sciences

Table of contents 

1. Measuring Freezing Tolerance: Survival and Regrowth Assays

Daniel Z. Skinner and Kimberly Garland-Campbell


2. Measuring Freezing Tolerance: Electrolyte Leakage and Chlorophyll Fluorescence Assays

Anja Thalhammer, Dirk K. Hincha and Ellen Zuther


3. Conducting Field Trials for Freezing Tolerance Breeding in Cereals

 Luigi Cattivelli


4. A Whole Plant Screening Test to Identify Genotypes with Superior Freezing Tolerance

Annick Bertrand, Yves Castonguay and Josée Bourassa


5. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation

Evelyne Téoulé and Carine Géry


6. Common Garden Experiments to Characterize Cold Acclimation Responses in Plants from Different Climatic Regions

Andrey Malyshev, Hugh A. L. Henry and Juergen Kreyling


7. Identification of Arabidopsis Mutants with Altered Freezing Tolerance

Carlos Perea-Resa and Julio Salinas


8. Infrared Thermal Analysis of Plant Freezing Processes

Gilbert Neuner and Edith Kuprian


9. Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

Seizo Fujikawa and Keita Endoh


10. Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images

David P. Livingston III and Tan D. Tuong


11. Proteomic Approaches to Identify Cold Regulated Soluble Proteins 

Stefanie Döll, Rico Lippmann and Hans-Peter Mock


12. Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins

 Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura and Matsuo Uemura


13. Profiling Methods to Identify Cold Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry

 Frederik Dethloff, Alexander Erban, Isabel Orf, Jessica Alpers, Ines Fehrle, Olga Beine-Golovchuk, Stefanie Schmidt, Jens Schwachtje and Joachim Kopka


14. A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition

Hieu Sy Vu, Sunitha Shiva, Aaron Smalter Hall and Ruth Welti


15. Quantification of Superoxide and Hydrogen Peroxide in Leaves

Ilona Juszczak and Margarete Baier


16. Estimating Ice Encasement Tolerance of Herbage Plants

Bjarni E. Gudleifsson and Brynhildur Bjarnadottir


17. Characterization of Ice Binding Proteins from Sea Ice Algae

 Maddalena Bayer-Giraldi, EonSeon Jin and Peter W. Wilson


18. Isolation and Characterization of Ice Binding Proteins from Higher Plants Adam J. Middleton, Barbara Vanderbeld, Melissa Bredow, Heather Tomalty, Peter L. Davies and Virginia K. Walker

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