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Life Sciences - Plant Sciences | Flower Development - Methods and Protocols

Flower Development

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1110

Riechmann, José Luis, Wellmer, Frank (Eds.)

2014, XIV, 475 p. 64 illus., 31 illus. in color.

A product of Humana Press
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  • Includes cutting-edge methods and protocols in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

In Flower Development: Methods and Protocols, researchers in the field detail protocols for  experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls



Authoritative and practical, Flower Development: Methods and Protocols  is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.

Content Level » Professional/practitioner

Keywords » experimental systems - flower development - flower development studies - genetic methods - genomics and systems biology - microscopy and histology - molecular and genetic studies - phenotypic analyses

Related subjects » Plant Sciences

Table of contents 

Part I: Review and Overview Chapters

1. Flower Development in Arabidopsis - There is More to it than Learning your ABCs

Nathanael Prunet and Thomas Jack


2. Flower Development in the Asterid Lineage

Barry Causier and Brendan Davies


3. Grass Flower Development

Hiro-Yuki Hirano, Wakana Tanaka, and Taiyo Toriba


4. Flower Diversity and Angiosperm Diversification

Pamela S. Soltis and Douglas E. Soltis


5. Flower Development - Open Questions and Future Directions

Frank Wellmer, John L. Bowman, Brendan Davies, Cristina Ferrándiz, Jennifer C. Fletcher, Robert G. Franks, Emmanuelle Graciet, Veronica Gregis, Toshiro Ito, Thomas P. Jack, Yuling Jiao, Martin M. Kater, Hong Ma, Elliot M. Meyerowitz, Nathanael Prunet, José Luis Riechmann


PART II: Genetic and Phenotypic Analyses

6. Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors

Thanh Theresa Dinh, Elizabeth Luscher, Shaofang Li, Xigang Liu, So Youn Won, and Xuemei Chen


7. Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development

Mona M. Monfared and Jennifer C. Fletcher


8. Genetic and Phenotypic Analyses of Petal Development in Arabidopsis

Judit Szécsi, Barbara Wippermann and Mohammed Bendahmane



9. Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsisand Rice

Fang Chang, Zaibao Zhang, Yue Jin, and Hong Ma


10. Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana

Yingxiang Wang, Zhihao Cheng, Pingli Lu, Ljudmilla Timofejeva, Hong Ma


11. Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis

Vicente Balanzà, Patricia Ballester, Monica Colombo, Chloé Fourquin, Irene Martínez-Fernández, and Cristina Ferrándiz




Part III: Microscopy and Histology

12. Microscopic Analysis of Arabidopsis Ovules

Balaji Enugutti and Kay Schneitz


13. Scanning Electron Microscopy Analysis of Floral Development

Robert G. Franks


14. Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue

Anna Medzihradszky, Kay Schneitz, and Jan U. Lohmann


15. The GUS Reporter System in Flower Development Studies

Janaki S. Mudunkothge and Beth A. Krizek



Part IV: Experimental Systems

16. A Floral Induction System for the Study of Early Arabidopsis Flower Development

Diarmuid Ó’Maoiléidigh and Frank Wellmer


17. Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types

G. Venugopala Reddy


18. Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers

Ying Wang and Yuling Jiao


19. Laser-assisted Microdissection Applied to Floral Tissues

Samuel E. Wuest and Ueli Grossniklaus


PartV: Molecular Biology, Genomics, and Systems Biology


20. Identification of Arabidopsis Knock-out Lines for Genes of Interest

José Tomás Matus, Thilia Ferrier, and José Luis Riechmann


21. Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues

Mariana Bustamante, Jian Jin, Oriol Casagran, Tania Nolan, and José Luis Riechmann


22. Misexpression Approaches for the Manipulation of Flower Development

Yifeng Xu, Eng-Seng Gan, and Toshiro Ito


23. Next Generation Sequencing Applied to Flower Development: RNA-Seq

Jun He and Yuling Jiao


24. Next-Generation Sequencing Applied to Flower Development: ChIP-Seq

Emmanuelle Graciet, Diarmuid Seosamh Ó'Maoiléidigh, and Frank Wellmer

 25. Live-Imaging of the Arabidopsis Inflorescence MeristemMarcus G. Heisler and Carolyn Ohno


26. Gene Regulatory Network Models for Floral Organ Determination

Eugenio Azpeitia, José Davila-Velderrain, Carlos Villarreal, and Elena R. Alvarez-Buylla


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Distribution rights for India: Delhi Book Store, New Delhi, India

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