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Life Sciences - Plant Sciences | Protocol for Somatic Embryogenesis in Woody Plants

Protocol for Somatic Embryogenesis in Woody Plants

Series: Forestry Sciences, Vol. 77

Jain, Shri Mohan, Gupta, Pramod K. (Eds.)

2005, X, 590 p.

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Earlier, we edited a well received series on "Somatic embryogenesis in woody trees", volumes 1 to 6. These volumes provided readers extensive reviews on somatic embryogenesis of important angiosperm and gymnosperm tree species; an excellent source of information for newcomers or those already engaged in research. However, these book volumes did not cover stepwise "detailed protocols" for inducing somatic embryogenesis. This book contains 46 chapters, divided into 4 sections: A) 12 chapters on conifers, B) 14 chapters on fruits, C) 14 chapters on angiosperms, and D) 6 chapters on histology, bioencapsulation, protoplasts, cryopreservation, double staining and thin cell layer sectioning. The book provides stepwise protocols for somatic embryogenesis of a range of selected woody plants in order to assist researchers to initiate somatic embryogenic cultures without too much alterations in protocols. Each chapter provides information on initiation and maintenance of embryogenic cultures; somatic embryo development, maturation, and germination; acclimitization and field transfer of somatic seedlings. Some chapteres include applications of somatic embryogenesis cultures, e.g. protoplasts, encapsulation, cryopreservation, genetic transformation, genetic fidelity with molecular markers, and bioreactor.

Content Level » Research

Keywords » Bur - Embryo - conifers - genetic transformation - olive - seedlings - woody plants

Related subjects » Forestry - Life Sciences - Plant Sciences

Table of contents 

Section A: 1. Slash pine (Pinus elliottii Engelm.), R.J. Newton, et al. 2. Somatic embryogenesis and genetic transformation in Pinus radiate, C. Walters, J.I. Find. 3. Douglas–fir (Pseudotsuga menziesii), P.K. Gupta, D. Holmstrom. 4. Omorika spruce (Picea omorika), S. Mihaljevic, S. Jelaska. 5. Somatic embryogenesis in Picea glauca, E.C. Young, T.A. Thorpe. 6. Protocol of somatic embryogenesis: Black spruce (Picea mariana (Mills.)B.S.P.), F.M. Tremblay, et al. 7. Sitka spruce (Picea sitchensis), D. Thompson, F. Harrington. 8. Protocol of somatic embryogenesis of Pinus nigra Arn, T. Salajova, et al. 9. Loblolly pine (Pinus taeda), W. Tang, R.J. Newton. 10. Somatic embryogenesis in maritime pine (Pinus pinaster Ait.) L. Harvengt. 11. Somatic embryogenesis in Pinus patula, C.S. Ford, et al. 12. Somatic embryogenesis in Norway spruce, M. Vagner, et al. Section B: 13. Cashew (Anacardium occidentale L.), R.S. Nadgauda, S.S. Gogate. 14. Somatic embryogenesis protocol: coffee (Coffea arabica L. and C. canephora P.), H. Etienne. 15. Protocols for somatic embryogenesis and plantlet formation from three explants in tea (Camellia sinensis (l.) o. kuntze), A. Akula, C. Akula. 16. Protocol of somatic embryogenesis from Citrus spp. anther culture, M.A. Germana. 17. Integration system for propagation of Theobroma cacao L., S.N. Maximova, et al. 18. Mango (Mangifera indica L.), H. Ara, et al. 19. Somatic embryogenesis in jackfruit (Artocarpus heterophyllus Lam.), S.K. Roy, R.K. Debnath. 20. Somatic embryogenesis in Indian olive (Elaeocarpus robustus L.), S.K. Roy, P. Sinha. 21. Rescue of endangered palms by in vitro methods: the case of ‘bottle palm’, V. Sarasan, et al. 22. Somatic embryogenesis in American grapes (Vitis x labruscana L.H. Bailey), S. Motoike, et al. 23. Pistachio, Pistachio vera L., A. Onay. 24. Grape (Vitis vinifera L.), D.K. Das, et al. 25. Date palm, Phoenix dactylifera L., J.M. Al-Khayri. 26. Somatic embryogenesis protocol: Citrus, F. Carimi.27. Olive (Olea europaea), M. Mencuccini, et al. Section C: 28. Protocol of somatic embryogenesis: Dalbergia sissoo Roxb. (Sissoo), A.K. Singh, S. Chand. 29. Protocol of somatic embryogenesis: Pedunculate oak (Quercus robur L.) and sessile oak (Quercus petraea /Matt./Liebl.), V. Chalupa. 30. Protocol of somatic embryogenesis: tamarillo (Cyphomandra betacea (Cav.) Sendtn.), J.M. Canhoto, et al. 31. Protocol of somatic embryogenesis: European chestnut (Castanea sativa Mill.), U. Sauer, E. Wilhelm. 32. Protocol of somatic embryogenesis in Acacia arabica (Lamk.) Willd., G.R. Rout, R.M. Nanda. 33. Protocol for hazelnut somatic embryogenesis, B. Berros, et al. 34. Protocol of somatic embryogenesis: Ocotea catharinensis Mez. (Lauraceae), A.M. Viana, et al.

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