Monitoring Antagonistic Fungi Deliberately Released into the Environment

1. Front Matter

   Pages i-xv

   PDF

2. Introduction

   1. Monitoring of Antagonistic Fungi. Perspectives, Needs and Legislation

      • J. M. Lynch

      Pages 1-9

3. Fungal Ecology

   1. Some Aspects of Fungal Ecology

      • Bengt Söderström

      Pages 11-16

   2. Quantification of Fungal Growth in the Environment

      • J. Schnürer, T. Börjesson

      Pages 17-24

   3. Population Growths and Survival of Trichoderma Harzianum and Trichoderma Virens in Sphagnum Peat

      • Nina Heiberg, Helge Green, Dan Funck Jensen

      Pages 25-31

   4. Occurrence of Gliocladium Roseum on Barley Roots in Sand and Field Soil

      • Inge M. B. Knudsen, Birgit Jensen, Dan Funck Jensen, John Hockenhull

      Pages 33-37

   5. Use of a Metalaxyl Tolerant Pythium Oligandrum Isolate for Selectively Following Its Population in Soil and Its Colonization of Roots of Diseased and Non-Diseased Plants

      • Anne Mette Madsen

      Pages 39-48

   6. Recovery and Detection of Deuteromycete Conidia from Soil

      • Susanne Elmholt, Helle Hestbjerg

      Pages 49-55

   7. The Prevalence of Fusarium Solani in Wrinkled and Round-Seeded Pea Genotypes

      • L. Bødker

      Pages 57-61

   8. Release of a Cord-Forming Basidiomycete Antagonistic to Heterobasidion Annosum and Its Subsequent Reisolation and Identification

      • L. Holmer, J. Stenlid

      Pages 63-70

   9. Methods to Quantify Nematophagus Fungi in Soil: Microscopy or Gus Gene Activity

      • Lotta Persmark, Yvonne Persson, Hans-Börje Jansson

      Pages 71-75

   10. Monitoring of a Gus Transformed Strain of Trichoderma Harzianum in Soil and Rhizosphere

       • Helge Green, Dan Funck Jensen

       Pages 77-83

   11. Monitoring growth of Bipolaris sorokiniana in plant tissue using GUS (β-glucuronidase) as a marker

       • E. Liljeroth

       Pages 85-89

4. Secondary Metabolites and Enzymes

   1. Identification of Fungi by Secondary Metabolites

      • Ulf Thrane

      Pages 91-98

   2. Prehelminthosporol, a Phytotoxin from Bipolaris Sorokiniana

      • H. Åkesson, H.-B. Jansson

      Pages 99-104

   3. Monitoring the Activity of Different Trichoderma Isolates by the Isoelectric Points (pI) of Their Extracellular Enzymes

      • C. Thrane, A. Tronsmo, D. F. Jensen

      Pages 105-111

5. DNA-Techniques

   1. PCR-Based Methods — A Promising Tool for Detection and Identification of Fungi in Soil

      • Mette Lubeck, Peter Stephensen Lubeck

      Pages 113-121

   2. Identification and Characterization of Isolates of Trichoderma and Gliocladium by PCR-Based Methods

      • Mette Lubeck, Sergey A. Bulat, Peter Stephensen Lubeck, Nina Mironenko, Dan Funck Jensen

      Pages 123-128

   3. Identification of Nematode-Trapping Fungi of the Genus Arthrobotrys Using RFLP Analysis of PCR-Amplified rDNA

      • Yvonne Persson, Susanne Erland, Hans-Börje Jansson

      Pages 129-132

   4. Cloning and Characterization of Trichoderma Harzianum Genes Induced During Growth on Rhizoctonia Solani Cell Walls

      • Valerie Vasseur, Marc Van Montagu, Gustavo H. Goldman

      Pages 133-137

Microorganisms, including those genetically modified (GMOs), are increasingly being released deliberately into the environment for different purposes. One major interest is the use of antagonistic organisms for biological control of pests and plant diseases. Both in the development of biocontrol agents and their later risk assessment and product approval, there is a need for methods that facilitate monitoring of such introduced microorganisms. This book particularly addresses the various approaches for monitoring introduced fungi and the current status of techniques which may be relevant to use are described. Such techniques include marker genes and other DNA-based techniques, serology using polyclonals or monoclonals, the use of enzymes or secondary metabolite profiles, and `electronic noses' as well as more traditional methods like dilution plating.

• Fungi
• development
• growth
• pea
• plant
• plants
• roots
• soil

• Department of Plant Biology, Plant Pathology Section, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark

  Dan Funck Jensen

• Department of Microbial Ecology, Lunds University, Lund, Sweden

  Hans-Börje Jansson

• Department of Biological Sciences, Agricultural University of Norway, ÅS-NLH, Norway

  Arne Tronsmo

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