Biophysical Techniques in Photosynthesis

1. Front Matter

   Pages i-xi

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2. Optical Methods

   1. Developments in Classical Optical Spectroscopy

      • Jan Amesz

      Pages 3-10

   2. Linear and Circular Dichroism

      • Győző Garab

      Pages 11-40

   3. Fluorescence

      • Kenneth Sauer, Martin Debreczeny

      Pages 41-61

   4. Ultrafast Spectroscopy of Photosynthetic Systems

      • Ralph Jimenez, Graham R. Fleming

      Pages 63-73

   5. Data Analysis of Time-Resolved Measurements

      • Alfred R. Holzwarth

      Pages 75-92

   6. Photosynthetic Thermoluminescence as a Simple Probe of Photosystem II Electron Transport

      • Yorinao Inoue

      Pages 93-107

   7. Accumulated Photon Echo Measurements of Excited State Dynamics in Pigment-Protein Complexes

      • Thijs J. Aartsma, Robert J.W. Louwe, Peter Schellenberg

      Pages 109-122

   8. Spectral Hole Burning: Methods and Applications to Photosynthesis

      • N. Raja S. Reddy, Gerald J. Small

      Pages 123-136

   9. Infrared and Fourier-Transform Infrared Spectroscopy

      • Werner Mäntele

      Pages 137-160

   10. Resonance Raman Studies in Photosynthesis — Chlorophyll and Carotenoid Molecules

       • Bruno Robert

       Pages 161-176

   11. Stark Spectroscopy of Photosynthetic Systems

       • Steven G. Boxer

       Pages 177-189

   12. The Photoacoustic Method in Photosynthesis — Monitoring and Analysis of Phenomena Which Lead to Pressure Changes Following Light Excitation

       • Shmuel Malkin

       Pages 191-206

3. Magnetic Resonance

   1. Magnetic Resonance: An Introduction

      • Arnold J. Hoff

      Pages 209-210

   2. Time-Resolved Electron Paramagnetic Resonance Spectroscopy — Principles and Applications

      • Haim Levanon

      Pages 211-233

   3. Electron Spin Echo Methods in Photosynthesis Research

      • R. David Britt

      Pages 235-253

   4. ENDOR Spectroscopy

      • Wolfgang Lubitz, Friedhelm Lendzian

      Pages 255-275

   5. Optically Detected Magnetic Resonance (ODMR) of Triplet States in Photosynthesis

      • Arnold J. Hoff

      Pages 277-298

   6. Magic Angle Spinning Nuclear Magnetic Resonance of Photosynthetic Components

      • Huub J.M. de Groot

      Pages 299-313

4. Structureand Oxygen

   1. Structure Determination of Proteins by X-Ray Diffraction

      • Marianne Schiffer

      Pages 317-324

Progress in photosynthesis research is strongly dependent on instrumentation. It is therefore not surpr- ing that the impressive advances that have been made in recent decades are paralleled by equally impressive advances in sensitivity and sophistication of physical equipment and methods. This trend started already shortly after the war, in work by pioneers like Lou Duysens, the late Stacy French, Britton Chance, Horst Witt, George Feher and others, but it really gained momentum in the seventies and especially the eighties when pulsed lasers, pulsed EPR spectrometers and solid-state electronics acquired a more and more prominent role on the scene of scientific research. This book is different from most others because it focuses on the techniques rather than on the scientific questions involved. Its purpose is three-fold, and this purpose is reflected in each chapter: (i) to give the reader sufficient insight in the basic principles of a method to understand its applications (ii) to give information on the practical aspects of the method and (iii) to discuss some of the results obtained in photosynthesis research in order to provide insight in its potentalities. We hope that in this way the reader will obtain sufficient information for a critical assessment of the relevant literature, and, perhaps more important, will gain inspiration to tackle problems in his own field of research. The book is not intended to give a comprehensive review of photosynthesis, but nevertheless offers various views on the exciting developments that are going on.

• ODMR
• Transport
• electron microscopy
• electron spin
• electron spin echo
• electron transport
• infrared spectroscopy
• light excitation
• optical spectroscopy
• photoacoustic
• photosynthesis
• photosynthetic thermoluminescence
• photosystem
• pigment-protein
• thermoluminescence

`The book is well presented and referenced with useful figures and should be used by all those interested in photosynthesis.'
Annals of Botany 80: 825-834 (1997)

• Department of Biophysics Huygens Laboratory, University of Leiden, Leiden, The Netherlands

  Jan Amesz, Arnold J. Hoff

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