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  • © 2013

Bacterial Cell Surfaces

Methods and Protocols

Editors:

  • Provides chapters contributed by highly recognized experts on a wide variety of topics that pertain to bacterial cell surfaces and the bacterial envelope
  • Features easy to follow methodologies that been proven successful in labs around the world
  • Includes key tips and detailed implementation advice to ensure accurate results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 966)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
  2. Experimental Manipulation of the Microbial Functional Amyloid Called Curli

    • Yizhou Zhou, Daniel R. Smith, David A. Hufnagel, Matthew R. Chapman
    Pages 53-75
  3. Visualization of Gram-positive Bacterial Pili

    • Chungyu Chang, I-Hsiu Huang, Antoni P. A. Hendrickx, Hung Ton-That
    Pages 77-95
  4. Single Cell Microfluidic Studies of Bacterial Motility

    • Adrien Ducret, Olivier Théodoly, Tâm Mignot
    Pages 97-107
  5. Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule

    • Susan M. Steenbergen, Eric R. Vimr
    Pages 109-120
  6. Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance

    • Elena B. Tikhonova, Helen I. Zgurskaya
    Pages 133-155
  7. Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion System

    • Tanya L. Johnson, Aleksandra E. Sikora, Ryszard A. Zielke, Maria Sandkvist
    Pages 157-172
  8. Pore Formation by T3SS Translocators: Liposome Leakage Assay

    • Eric Faudry, Caroline Perdu, Ina Attrée
    Pages 173-185
  9. Isolation of Bacterial Type IV Machine Subassemblies

    • Mayukh K. Sarkar, Seyyed I. Husnain, Simon J. Jakubowski, Peter J. Christie
    Pages 187-204
  10. Assembly of Bacterial Outer Membrane Proteins

    • Jan Grijpstra, Martine P. Bos, Jan Tommassen
    Pages 223-237
  11. The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and Characterization

    • Jessica V. Hankins, James A. Madsen, Brittany D. Needham, Jennifer S. Brodbelt, M. Stephen Trent
    Pages 239-258
  12. Quantitative and Qualitative Preparations of Bacterial Outer Membrane Vesicles

    • Halima Chutkan, Ian MacDonald, Andrew Manning, Meta J. Kuehn
    Pages 259-272
  13. In Vitro Peptidoglycan Synthesis Assay with Lipid II Substrate

    • Jacob Biboy, Nhat Khai Bui, Waldemar Vollmer
    Pages 273-288
  14. Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes

    • Filipe Carvalho, María Graciela Pucciarelli, Francisco García-del Portillo, Didier Cabanes, Pascale Cossart
    Pages 289-308

About this book

In recent years, molecular microbiology has emerged as a top, cutting-edge biological discipline, thanks to the multi-disciplinary and integrative approaches taken by investigators seeking to understand the intricacies of the microbial world and how it affects human health and the biosphere. In Bacterial Cell Surfaces: Methods and Protocols, recent advances in structural biology, proteomics, and imaging techniques, together with the traditional biochemical and genetic approaches, are provided in order to present an exciting look into the structure, function, and regulation of the bacterial cell envelope. The detailed volume contains examples of traditional and innovative tools for the study of protein structure and function and enzymatic activities, the purification and analysis of macromolecules and their complexes, and the investigation of regulatory mechanisms and cell biological processes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Bacterial Cell Surfaces: Methods and Protocols is aimed at the microbiologist, biochemist, or cell biologist, whether a beginning graduate student or a veteran experimentalist, who wishes to learn new methodologies and take advantage of the years of research and protocol optimization from the best laboratories around the world.

Editors and Affiliations

  • , Dept. of Biology and Biochemistry, University of Houston, Houston, USA

    Anne H. Delcour

Bibliographic Information

  • Book Title: Bacterial Cell Surfaces

  • Book Subtitle: Methods and Protocols

  • Editors: Anne H. Delcour

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-245-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-244-5Published: 09 January 2013

  • Softcover ISBN: 978-1-4939-5993-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-245-2Published: 10 January 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 399

  • Topics: Microbiology, Bacteriology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access