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Life Sciences - Microbiology | Helicobacter Species - Methods and Protocols

Helicobacter Species

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 921

Houghton, Jeanmarie (Ed.)

2013, X, 258 p. 28 illus., 8 illus. in color.

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  • Presents methods and protocols commonly used to study Helicobacter
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice form the experts

Identifying  Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states.  Over the last several decades, scientists have come to understand that the interplay between the bacteria, the host, and the environment all contribute to the clinical outcome of infection. In Helicobacter Species: Methods and Protocols, expert researchers in the field detail many of the methods and which are now commonly used to study Helicobacter infection. These include protocols and methods that have evolved over time, and standards across the field have been established which are essential for optimal outcomes and to allow comparison of data across different laboratories. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Helicobacter Species: Methods and Protocols seeks to aid scientists in further study of this crucially important research into Helicobacter research.

Content Level » Professional/practitioner

Keywords » In vivo studies - bacteria - bacterial virulence factors - gastric cancer - host epithelial cells - immune cells - infection

Related subjects » Microbiology

Table of contents 

1. Introduction           

JeanMarie Houghton

 

2.   Helicobacter pylori: An Overview

Jennifer M. Noto and Richard M. Peek, Jr.

 

3. Perspectives on Methodology for in vitro Culture of Helicobacter pylori

Timothy L. Cover

 

4. Successful Culture Techniques for Helicobacter Species : General Culture Techniques for Helicobacter pylori

Jeannette M. Whitmire and D. Scott Merrell

 

5. Successful Culture Techniques for Helicobacter Species: Establishing H. pylori Cultures From Infected Rodents

Jeannette M. Whitmire and D. Scott Merrell

 

6. Successful Culture Techniques for Helicobacter Species: Verification of Helicobacter Identity Using 16S rRNA Gene Sequence Analysis

Jeannette M. Whitmire and D. Scott Merrell

 

7. The Helicobacter pylori cag Pathogenicity Island

Jennifer M. Noto and Richard M. Peek, Jr.

 

8. Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori

Jennifer M. Noto and Richard M. Peek, Jr.

 

9. A Method for Short-term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori

Marina Leite and Ceu Figueiredo 10. Cell Culture Based Assays to Test for Bacterial Adherence and Internalization

Deepa Raju, David Rizzuti and Nicola L. Jones

 

11. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence

Deepa Raju, David Rizzuti, Nicola L. Jones

 

12. Rodent Models of Helicobacter Infection, Inflammation and Disease

 Songhua Zhang and  Steven F. Moss

 

13. Bacterial Culture and Inoculation of Mice (Simple Infection)

Brian M. Gray and Kathryn A. Eaton

 

14. Adoptive Transfer of Splenocytes to Immunocompromised Mice

Brian M. Gray and Kathryn A. Eaton

 

15. Isolation of Gastric Lamina Propria Leukocytes (LPL)

Brian M. Gray and Kathryn A. Eaton

 

16. Delayed-type Hypersensitivity Determination

Brian M. Gray and Kathryn A. Eaton

 

17. Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression

Brian M. Gray and Kathryn A. Eaton

 

18. Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse

Nancy S. Taylor and James G. Fox

 

19. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice

Mark T Whary, Zhongming Ge and James G Fox

 

20. Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes

Richard L. Ferrero, John E. Wilson, and Phil Sutton

 

21. Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections

Arlin B. Rogers

 

22. Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models

Arlin B. Rogers

 

23. Innate Immune Responses to Helicobacter pylori Infection- An Overview

Milan K. Patel, Melanie I. Trombly  and Evelyn A. Kurt-Jones

 

24. Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection

Milan K. Patel, Glennice N. Bowen, Anna M. Cerny and Evelyn A. Kurt-Jones

 

25. Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection

Michael Craig, Michael Schumacher and Yana Zavros

 

26. In vivo Measurement of Helicobacter pylori Infection

Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri Tazehkand

 10. Cell Culture Based Assays to Test for Bacterial Adherence and Internalization

Deepa Raju, David Rizzuti and Nicola L. Jones

 

11. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence

Deepa Raju, David Rizzuti, Nicola L. Jones

 

12. Rodent Models of Helicobacter Infection, Inflammation and Disease

 Songhua Zhang and  Steven F. Moss

 

13. Bacterial Culture and Inoculation of Mice (Simple Infection)

Brian M. Gray and Kathryn A. Eaton

 

14. Adoptive Transfer of Splenocytes to Immunocompromised Mice

Brian M. Gray and Kathryn A. Eaton

 

15. Isolation of Gastric Lamina Propria Leukocytes (LPL)

Brian M. Gray and Kathryn A. Eaton

 

16. Delayed-type Hypersensitivity Determination

Brian M. Gray and Kathryn A. Eaton

 

17. Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression

Brian M. Gray and Kathryn A. Eaton

 

18. Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse

Nancy S. Taylor and James G. Fox

 

19. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice

Mark T Whary, Zhongming Ge and James G Fox

 

20. Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes

Richard L. Ferrero, John E. Wilson, and Phil Sutton

 

21. Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections

Arlin B. Rogers

 

22. Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models

Arlin B. Rogers

 

23. Innate Immune Responses to Helicobacter pylori Infection- An Overview

Milan K. Patel, Melanie I. Trombly  and Evelyn A. Kurt-Jones

 

24. Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection

Milan K. Patel, Glennice N. Bowen, Anna M. Cerny and Evelyn A. Kurt-Jones

 

25. Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection

Michael Craig, Michael Schumacher and Yana Zavros

 

26. In vivo Measurement of Helicobacter pylori Infection

Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri Tazehkand

 

3. Perspectives on Methodology for in vitro Culture of Helicobacter pylori

Timothy L. Cover

 

4. Successful Culture Techniques for Helicobacter Species : General Culture Techniques for Helicobacter pylori

Jeannette M. Whitmire and D. Scott Merrell

 

5. Successful Culture Techniques for Helicobacter Species: Establishing H. pylori Cultures From Infected Rodents

Jeannette M. Whitmire and D. Scott Merrell

 

6. Successful Culture Techniques for Helicobacter Species: Verification of Helicobacter Identity Using 16S rRNA Gene Sequence Analysis

Jeannette M. Whitmire and D. Scott Merrell

 

7. The Helicobacter pylori cag Pathogenicity Island

Jennifer M. Noto and Richard M. Peek, Jr.

 

8. Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori

Jennifer M. Noto and Richard M. Peek, Jr.

 

9. A Method for Short-term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori

Marina Leite and Ceu Figueiredo 10. Cell Culture Based Assays to Test for Bacterial Adherence and Internalization

Deepa Raju, David Rizzuti and Nicola L. Jones

 

11. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence

Deepa Raju, David Rizzuti, Nicola L. Jones

 

12. Rodent Models of Helicobacter Infection, Inflammation and Disease

 Songhua Zhang and  Steven F. Moss

 

13. Bacterial Culture and Inoculation of Mice (Simple Infection)

Brian M. Gray and Kathryn A. Eaton

 

14. Adoptive Transfer of Splenocytes to Immunocompromised Mice

Brian M. Gray and Kathryn A. Eaton

 

15. Isolation of Gastric Lamina Propria Leukocytes (LPL)

Brian M. Gray and Kathryn A. Eaton

 

16. Delayed-type Hypersensitivity Determination

Brian M. Gray and Kathryn A. Eaton

 

17. Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression

Brian M. Gray and Kathryn A. Eaton

 

18. Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse

Nancy S. Taylor and James G. Fox

 

19. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice

Mark T Whary, Zhongming Ge and James G Fox

 

20. Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes

Richard L. Ferrero, John E. Wilson, and Phil Sutton

 

21. Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections

Arlin B. Rogers

 

22. Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models

Arlin B. Rogers

 

23. Innate Immune Responses to Helicobacter pylori Infection- An Overview

Milan K. Patel, Melanie I. Trombly  and Evelyn A. Kurt-Jones

 

24. Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection

Milan K. Patel, Glennice N. Bowen, Anna M. Cerny and Evelyn A. Kurt-Jones

 

25. Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection

Michael Craig, Michael Schumacher and Yana Zavros

 

26. In vivo Measurement of Helicobacter pylori Infection

Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri Tazehkand

 10. Cell Culture Based Assays to Test for Bacterial Adherence and Internalization

Deepa Raju, David Rizzuti and Nicola L. Jones

 

11. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence

Deepa Raju, David Rizzuti, Nicola L. Jones

 

12. Rodent Models of Helicobacter Infection, Inflammation and Disease

 Songhua Zhang and  Steven F. Moss

 

13. Bacterial Culture and Inoculation of Mice (Simple Infection)

Brian M. Gray and Kathryn A. Eaton

 

14. Adoptive Transfer of Splenocytes to Immunocompromised Mice

Brian M. Gray and Kathryn A. Eaton

 

15. Isolation of Gastric Lamina Propria Leukocytes (LPL)

Brian M. Gray and Kathryn A. Eaton

 

16. Delayed-type Hypersensitivity Determination

Brian M. Gray and Kathryn A. Eaton

 

17. Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression

Brian M. Gray and Kathryn A. Eaton

 

18. Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse

Nancy S. Taylor and James G. Fox

 

19. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice

Mark T Whary, Zhongming Ge and James G Fox

 

20. Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes

Richard L. Ferrero, John E. Wilson, and Phil Sutton

 

21. Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections

Arlin B. Rogers

 

22. Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models

Arlin B. Rogers

 

23. Innate Immune Responses to Helicobacter pylori Infection- An Overview

Milan K. Patel, Melanie I. Trombly  and Evelyn A. Kurt-Jones

 

24. Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection

Milan K. Patel, Glennice N. Bowen, Anna M. Cerny and Evelyn A. Kurt-Jones

 

25. Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection

Michael Craig, Michael Schumacher and Yana Zavros

 

26. In vivo Measurement of Helicobacter pylori Infection

Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri Tazehkand

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