Overview
- Editors:
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Tuofu Zhu
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Departments of Laboratory Medicine and Microbiology, University of Washington School of Medicine, Seattle
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Table of contents (37 protocols)
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Methods for Virus Isolation and Detection
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- Janet D. Siliciano, Robert F. Siliciano
Pages 3-15
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- Hanneke Schuitemaker, Neeltje A. Kootstra
Pages 17-24
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- Paul R. Gorry, Secondo Sonza, Katherine Kedzierska, Suzanne M. Crowe
Pages 25-33
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- Santhi Gorantla, Myhanh Che, Howard E. Gendelman
Pages 35-48
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- Kathleen Borgmann, Howard E. Gendelman, Anuja Ghorpade
Pages 49-70
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- Michael D. Lairmore, Andy Montgomery
Pages 113-123
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- Joëlle Tobaly-Tapiero, Patricia Bittoun, Ali Saïb
Pages 125-137
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- Audrey Brussel, Olivier Delelis, Pierre Sonigo
Pages 139-154
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- Olivier Delelis, Audrey Brussel, Pierre Sonigo
Pages 155-170
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- Alcina Nicol, Gerard J. Nuovo
Pages 171-182
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- Kenji Kashima, Tsutomu Daa, Shigeo Yokoyama
Pages 183-189
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- Marie Gueudin, Florence Damond, François Simon
Pages 215-220
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- Marie Gueudin, François Simon
Pages 221-228
About this book
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.
Reviews
"More than 20 years after the discoveries of HTLV and HIV, there appears to be a need for a consolidated reference of many of the standard and not-so-standard protocols used to characterize the human retroviruses. This book, containing some 37 detailed protocols provided by multinational authors in two parts, does an admirable job. Part I, therefore, includes guidance on isolation of HTLV, HIV and Human Foamy Virus (HFV) from peripheral blood, lymph nodes, CNS tissue and genital secretions (semen, vaginal fluids). It also describes a variety of molecular methods (largely PCR-based) to assess viral levels, integration status and drug sensitivity, plus quantification by antigen detection. At some 313 pages, Part I forms the substantial portion of the book. Part II (168 pages) guides on assessing cell and receptor tropism, construction and utilization of molecular clones and also use of microarrays to look at host and viral gene expression. The book on the whole is well illustrated with clear photographs and diagrams to support the detailed methodology, and the references at the end of each protocol are comprehensive. "-SGM Quarterly
Editors and Affiliations
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Departments of Laboratory Medicine and Microbiology, University of Washington School of Medicine, Seattle
Tuofu Zhu
