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Life Sciences - Microbiology | Sepsis - Diagnostic Methods and Protocols

Sepsis

Diagnostic Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1237

Mancini, Nicasio (Ed.)

2015, XIII, 262 p. 27 illus., 18 illus. in color.

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

This volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches. Sepsis is a major clinical problem that takes an inordinate toll on human lives and economical resources. It is widely recognized that inappropriate treatment is associated with a dramatic  increase in mortality, especially within the first hours, therefore clinical and microbiological diagnosis are of pivotal importance in the management of septic patients. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Sepsis: Diagnostic Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this life-threatening illness.

Content Level » Professional/practitioner

Keywords » biomarkers - blood culture - broad-range PCR - fungal DNA - pathogens

Related subjects » Microbiology - Pathology

Table of contents 

1. Microbiological Diagnosis of Sepsis: The Confounding Effect of a "Gold Standard"

            Nicasio Mancini, Roberto Burioni, and Massimo Clementi

 

2. Pathopysiological Aspects of Sepsis: An Overview

            Ying-Yi Luan, Yong-Ming Yao, Qing-Hong Zhang, and Zhi-Yong Sheng

 

3. Clinical Aspects of Sepsis: An Overview

            Giacomo Monti, Giovanni Landoni, Daiana Taddei, Francesca Isella, and Alberto Zangrillo

 

4. Technical Improvements in Culturing Blood

            Giacomo Pardini

 

5. Matrix-Assisted Laser-Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF/MS)-Based Identification of Pathogens from Positive Blood Culture Bottles

            Philippe Lagacé-Wiens

           

6. Bacterial and Fungal DNA Extraction from Positive Blood Culture Bottles: A Manual and an Automated Protocol

            Minna Mäki

 

7. Broad-Range-PCR in the Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Sequencing Approach

            Yoshitomo Morinaga and Katsunori Yanagihara

 

8. Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Microarray-Based Approach

            Teresa Raich and Scott Powell

 

9. Detection of Carbapenemases Using Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Meropenem Hydrolysis Assay

            Jroslav Hrabák

 

10.  Molecular Detection of Antibiotic Resistance Genes from Positive Blood Cultures

            Musa Y. Hindiyeh, Gill Smollan, Shiraz Gefen-Halevi, Ella Mendelson, and Nathan Keller

 

11. Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols

            Michael G. Lorenz, Helge Mühl, and Claudia Disqué

 

12. Bacterial and Fungal DNA Extraction from Blood Samples: Automated Protocols

            Michael G. Lorenz, Claudia Disqué, and Helge Mühl

 

13. Broad-Range-PCR in the Identification of Bacterial and Fungal Pathogens from Blood Samples: A Sequencing Approach

            Eva Leitner and Harald H. Kessler

 

14. Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples

            Madeleine Mai, Iris Müller, Daniela Maneg, Achim Haecker, Gerd Haberhausen, and Klaus-Peter Hunfeld

 

15. Host Response Biomarkers in the Diagnosis of Sepsis: A General Overview

            Marianna Parlato and Jean-Marc Cavaillon

 

16. Host Response Biomarkers in Sepsis: The Role of Procalcitonin

            Jean-Louis Vincent, Marc Van Nuffelen, and Christophe Lelubre

 

17. Host Response Biomarkers in Sepsis: Overview on sTREM-1 Detection

            Jérémie Lemarié, Damien Barraud, and Sébastien Gibot

 

18. Host Response Biomarkers in Sepsis: suPAR Detection

            Evangelos J. Giamarellos-Bourboulis and Marianna Georgitsi

 

19. Clinical Diagnosis of Sepsis and the Combined Use of Biomarkers, Culture- and Non-Culture-Based Assays

            Frank Bloos

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