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  • © 2015

Bacterial Pangenomics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1231)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
  2. Pulsed Field Gel Electrophoresis and Genome Size Estimates

    • Rosa Alduina, Annalisa Pisciotta
    Pages 1-14
  3. Choice of Next-Generation Sequencing Pipelines

    • F. Del Chierico, M. Ancora, M. Marcacci, C. Cammà, L. Putignani, Salvatore Conti
    Pages 31-47
  4. Bacterial Metabarcoding by 16S rRNA Gene Ion Torrent Amplicon Sequencing

    • Elio Fantini, Giulio Gianese, Giovanni Giuliano, Alessia Fiore
    Pages 77-90
  5. The Illumina-Solexa Sequencing Protocol for Bacterial Genomes

    • Zhenfei Hu, Lei Cheng, Hai Wang
    Pages 91-97
  6. High-Throughput Phenomics

    • Carlo Viti, Francesca Decorosi, Emmanuela Marchi, Marco Galardini, Luciana Giovannetti
    Pages 99-123
  7. Comparative Analysis of Gene Expression: Uncovering Expression Conservation and Divergence Between Salmonella enterica Serovar Typhimurium Strains LT2 and 14028S

    • Paolo Sonego, Pieter Meysman, Marco Moretto, Roberto Viola, Kris Laukens, Duccio Cavalieri et al.
    Pages 125-135
  8. Raw Sequence Data and Quality Control

    • Giovanni Bacci
    Pages 137-149
  9. Methods for Assembling Reads and Producing Contigs

    • Valerio Orlandini, Marco Fondi, Renato Fani
    Pages 151-161
  10. Mapping Contigs Using CONTIGuator

    • Marco Galardini, Alessio Mengoni, Marco Bazzicalupo
    Pages 163-176
  11. Gene Calling and Bacterial Genome Annotation with BG7

    • Raquel Tobes, Pablo Pareja-Tobes, Marina Manrique, Eduardo Pareja-Tobes, Evdokim Kovach, Alexey Alekhin et al.
    Pages 177-189
  12. Defining Orthologs and Pangenome Size Metrics

    • Emanuele Bosi, Renato Fani, Marco Fondi
    Pages 191-202
  13. Genome-Scale Metabolic Network Reconstruction

    • Marco Fondi, Pietro Liò
    Pages 233-256
  14. From Pangenome to Panphenome and Back

    • Marco Galardini, Alessio Mengoni, Stefano Mocali
    Pages 257-270
  15. The Integrated Microbial Genome Resource of Analysis

    • Alice Checcucci, Alessio Mengoni
    Pages 289-295

About this book

Bacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science. Thanks to the large diffusion of bacterial genome analysis, Bacterial Pangenomics: Methods and Protocols is able to provide the most recent methodologies about the study of bacterial pangenomes by covering the three major areas: the experimental methods for approaching bacterial pangenomics, the bio informatic pipelines for analysis and annotation of sequence data and finally the methods for inferring functional and evolutionary features from the pangenome. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Bacterial Pangenomics: Methods and Protocols will serve as a field guide for both qualified bacterial genomics investigators who want to update their technical knowledge, for less experienced researchers who want to start working with bacterial genomics and pangenomics, as well as serving as a manual and supplemental textbook for graduate students of genomics and bioinformatics.

Editors and Affiliations

  • Department of Biology, University of Florence, Florence, Italy

    Alessio Mengoni, Marco Fondi

  • EMBL-EBI, Cambridge, United Kingdom

    Marco Galardini

Bibliographic Information

  • Book Title: Bacterial Pangenomics

  • Book Subtitle: Methods and Protocols

  • Editors: Alessio Mengoni, Marco Galardini, Marco Fondi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1720-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1719-8Published: 24 October 2014

  • Softcover ISBN: 978-1-4939-5547-3Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1720-4Published: 24 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 298

  • Number of Illustrations: 17 b/w illustrations, 42 illustrations in colour

  • Topics: Microbiology, Bacteriology, Genetic Engineering

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access