Single-Pollen Genotyping

1. Front Matter

   Pages i-xi

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2. Significance of Single-Pollen Genotyping in Ecological Research

   • Yuji Isagi

   Pages 1-6

3. Procedure for Single-Pollen Genotyping

   • Yoshihisa Suyama

   Pages 7-15

4. Pollination Efficiencies of Insects Visiting Magnolia obovata, as Determined by Single-Pollen Genotyping

   • Yu Matsuki, Motoshi Tomita, Yuji Isagi

   Pages 17-32

5. Difference in Pollen Donor Composition During the Early Phases of Reproduction as Revealed by DNA Analysis of Pollen Grains and Seeds in the Monoecious Tree Castanea crenata

   • Yoichi Hasegawa, Yoshihisa Suyama, Kenji Seiwa

   Pages 33-46

6. Expanded Home Range of Pollinator Birds Facilitates Greater Pollen Flow of Camellia japonica in a Forest Heavily Damaged by Volcanic Activity

   • Harue Abe, Saneyoshi Ueno, Yoshihiko Tsumura, Masami Hasegawa

   Pages 47-62

7. Can Tiny Thrips Provide Sufficient Pollination Service During a General Flowering Period in Tropical Rainforest?

   • Toshiaki Kondo, Sen Nishimura, Yoko Naito, Yoshihiko Tsumura, Toshinori Okuda, Kevin Kit Siong Ng et al.

   Pages 63-81

8. Alien Dandelions Displace a Native Related Species Through Interspecific Pollen Transfer

   • Takashi Matsumoto, Koh-ichi Takakura, Takayoshi Nishida

   Pages 83-99

9. Single-Pollen Genotyping of Holocene Lake Sediments

   • Laura Parducci, Yoshihisa Suyama

   Pages 101-109

10. Potential Application of Pollen Genotyping for Evolutionary Genetic and Genomic Studies: Linkage/Recombination Analysis and Haplotype Sequencing

    • Yayoi Takeuchi, Kentaro K. Shimizu

    Pages 111-123

11. Back Matter

    Pages 125-127

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Pollination is one of the most important processes in plant reproduction. It directly influences reproductive success and fitness and the genetic structure of the plant population. Methods exist to infer the pattern and distance of pollen dispersal, but direct observation of the movements of individual pollen grains during pollination is not feasible owing to their small size. Single-pollen genotyping is a novel technique for genotyping a single pollen grain. In this book, the principles, the experimental protocol, and several applications of this method in studies of plant ecology, reproductive biology, and evolutionary genetics have been described. More specifically, the information is useful for the analysis of linkage disequilibrium, intraspecific genetic variation, chromosome mapping, and the origins of polyploidy. It is also essential for achieving sustainable and optimal crop yield and is vital to agriculture and forestry. Written by pioneer researchers, the book provides novel research approaches that are proving useful in a growing number of fields. This volume is intended to encourage new and continued applications of single-pollen genotyping among many disciplines in the future.

• Pollination
• conservation genetics
• gene flow
• microsatellite
• plant reproduction

From the reviews:

“Patterns of pollen dispersal and probabilities of cross pollination are possible to predict but this short book (127 pages) looks at a useful new technique whereby the genotype of single pollen grains can be identified. … this technique allowed the researchers to not only identify whether pollen was being carried from species to species but also to estimate how much of the pollen on a flower was from the same trees or from others, and hence the potential degree of self pollination.” (Peter Thomas, Bulletin of the British Ecological Society, Vol. 42 (2), 2011)

• Graduate School of Agriculture, Kyoto University, Kyoto, Japan

  Yuji Isagi

• Graduate School of Agricultural Science, Tohoku University, Osaki, Japan

  Yoshihisa Suyama

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