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Antibody Engineering

  • Book
  • © 2001

Overview

Editors:
  1. Roland Kontermann

    1. Institut für Molekularbiologie und Tumorforschung, Universität Marburg, Marburg, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Stefan Dübel

    1. Molekulare Genetik, Universität Heidelberg, Heidelberg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Comprehensive collection of proven lab protocols in the expanding field of antibody engineering
  • Beginners are introduced to the basic methods with detailed instructions
  • Professional antibody engineers find the latest approaches and emerging techniques
  • Each protocol is provided by an expert in this technology

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (50 chapters)

  1. Front Matter

    Pages I-XII
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Recombinant Antibodies

      • Stefan Dübel, Roland E. Kontermann
      Pages 3-16

  3. Generation of Recombinant Antibodies from Hybridoma

    1. Front Matter

      Pages 17-17
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    2. Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly

      • Jörg Burmester, Andreas Plückthun
      Pages 19-40

    3. Construction of scFv from Hybridoma by Two-Step Cloning

      • Frank Breitling, Dieter Moosmayer, Bodo Brocks, Stefan Dübel
      Pages 41-55

    4. Cloning Hybridoma cDNA by RACE

      • Andrew Bradbury
      Pages 56-61

  4. Generation of Antibody Libraries

    1. Front Matter

      Pages 63-63
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    2. Phage — Display Libraries of Murine Antibody Fab Fragments

      • Jan Engberg, Liselotte Brix Jensen, Ali Fazil Yenidunya, Katja Brandt, Erik Riise
      Pages 65-92

    3. Generation of Naive Human Antibody Libraries

      • Catherine Hutchings, Sara Carmen, Simon Lennard
      Pages 93-108

    4. Generation of Antibody Gene Libraries from Seropositive Human Donors

      • Joachim Koch, Stefan Dübel
      Pages 109-123

    5. Generation of Rabbit Immune Libraries

      • Rüdiger Ridder, Hermann Gram
      Pages 124-134

  5. Isolation of Antibody Fragments from Combinatorial Libraries

    1. Front Matter

      Pages 135-135
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    2. Immunotube Selections

      • Roland E. Kontermann
      Pages 137-148

    3. Selections on Biotinylated Antigens

      • Patrick Chames, Hennie R. Hoogenboom, Paula Henderikx
      Pages 149-166

    4. Guided Selection on Cells

      • Silvana Canevari, Mariangela Figini
      Pages 167-183

    5. Proximity (ProxiMol) and Step-Back Selections

      • Jane K. Osbourn
      Pages 184-192

    6. Selections on Tissue Sections

      • Jesper Max Tordsson, Thomas Niklas Brodin, Pia Jasmine Karlström
      Pages 193-205

    7. Selection of Human Antibody Fragments by Anti-Idiotypic Interaction

      • Peter Fischer
      Pages 206-212

    8. Selecting Intracellular Antibodies Using the Two-Hybrid System

      • Michela Visintin, Antonino Cattaneo
      Pages 213-233
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Keywords

About this book

Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more.
This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered.

Editors and Affiliations

  • Institut für Molekularbiologie und Tumorforschung, Universität Marburg, Marburg, Germany

    Roland Kontermann

  • Molekulare Genetik, Universität Heidelberg, Heidelberg, Germany

    Stefan Dübel

Bibliographic Information

  • Book Title: Antibody Engineering

  • Editors: Roland Kontermann, Stefan Dübel

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-662-04605-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2001

  • eBook ISBN: 978-3-662-04605-0Published: 29 June 2013

  • Edition Number: 1

  • Number of Pages: XII, 790

  • Number of Illustrations: 116 b/w illustrations, 9 illustrations in colour

  • Topics: Cell Biology, Immunology, Biotechnology, Biochemistry, general

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